毛脉酸模的组织培养及植株再生

以毛脉酸模下胚轴和幼苗根茎为外植体，以MS为基本培养基，与不同浓度比例的6-BA和2,4-D等植物生长调节剂进行毛脉酸模组织培养的研究。结果表明，毛脉酸模幼苗根茎是诱导丛生芽的最佳材料，诱导丛生芽产生的最佳培养基为MS+6-BA 3.0 mg·L^-1+2,4-D 0.1 mg·L^-1，诱导率为81.2%；生根培养基为无激素的MS培养基，生根率可达92%，在此条件下生根迅速，可获得完整植株；组培苗经过炼苗移栽后成活率可达80%。

Tissue Culture and Plant Regeneration of Rumex gmelini Turcz.

With the hypocotyls and seedling rhizomes of Rumex gmelini Turcz. as explants, the tissue culture of R.gmelini was studied by adding different concentrations of 6-BA and 2,4-D into MS basic mediums. The results showed that the better materials for multiple shoot clumps induction were seedling rhizomes; the best medium for multiple shoot clumps induction was MS+6-BA 3.0 mg·L^-1+2,4-D 0.1 mg·L^-1, the induction rate was 81.2%; the rooting medium was MS basic mediums without hormones, the rooting rate could get 92%, and under this conditions the plant regeneration could be obtained rapidly. After transplantation, about 80% of these seedlings could survive.