小果卫矛愈伤组织诱导及植株再生体系的建立

以小果卫矛嫩茎为外植体,采用L 9(34)正交设计法,研究了不同灭菌组合、不同基本培养基、不同浓度6-BA、2,4-D、NAA配比对小果卫矛愈伤组织诱导、再分化及生根的影响。结果表明:小果卫矛嫩茎最适的灭菌组合为75%酒精消毒30 s+0.1%升汞消毒15 min,愈伤组织诱导的最佳培养基为MS+3.0 mg·L-16-BA+1.0 mg·L-12,4-D,诱导率为79%;再分化的最佳培养基为MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1,再分化率为78.83%,1/2 MS+NAA 1.2 mg·L-1适用于生根培养,生根率达到83.23%。

Callus induction and Plant Regeneration of Euonymus microcarpus(Oliv.) Spraggue

With the young stems of Euonymus microcarpus(Oliv.) Spraggue as explants, L₉(3⁴) orthogonal test was used to study the effects of different sterilization combinations and different basal medium and combinations of different plant growth regulator on the induction and regeneration and rooting of callus. The most appropriate method of surface sterilization on leaves was with 75% alcohol for 30 s, and then 0.1% HgCl₂ for 15 min, the optimal medium for callus induction was MS containing 6-BA 3.0 mg·L^-1 and 2,4-D 1.0 mg·L^-1 with a maximum induction rate of 79%, the optimum medium for shoot differentiation was MS containing 6-BA 2.0 mg·L^-1 and NAA 0.2 mg·L^-1 with 78.83% of differentiation rate, and the suitable rooting medium was 1/2MS containing NAA 1.2 mg·L^-1 with 83.23% of rooting rate.