| 白桦肌动蛋白(Actin)基因全长cDNA克隆与序列分析 |
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| 引用本文： | 陈鹏飞,刘雪梅,宋福南,宋兴舜,刘霓,金微微,刘威.白桦肌动蛋白(Actin)基因全长cDNA克隆与序列分析[J].植物研究,2009,29(3):339-345. |
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| 作者姓名： | 陈鹏飞 刘雪梅 宋福南 宋兴舜 刘霓 金微微 刘威 |
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| 作者单位： | (1.东北林业大学生命科学学院，哈尔滨150040) ;(2.东北林业大学林木遗传育种与生物技术教育部重点实验室，哈尔滨150040) |
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| 基金项目： | 黑龙江省“十一五”科技攻关项目(No.GA06B301-2-3);;黑龙江省教育厅科技项目(No.11523003);;哈尔滨市科技创新人才研究专项资金项目(2008RFQXN009) |
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| 摘 要： | 以白桦(Betula platyphylla Suk.)次生木质部为材料，用改良CTAB方法提取总RNA。根据植物肌动蛋白(Actin)基因编码区的保守序列设计引物后进行RT-PCR，并采用RACE技术扩增出Actin基因全长序列。该基因cDNA全长1 785 bp，序列分析表明，该基因编码区1 134 bp，编码377个氨基酸，5′非编码区157 bp，3′非编码区495 bp。所得序列与GenBank中注册的其它植物肌动蛋白核苷酸序列的相似性均在80%以上，氨基酸序列的相似性高达96%以上。此基因已在GenBank注册（EU588981）。根据高等植物肌动蛋白相似性构建了进化树，表明白桦肌动蛋白与蓖麻肌动蛋白之间的亲缘关系最为密切，在进化中分化时间最为接近。
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| 关 键 词： | 白桦 肌动蛋白 基因克隆 序列分析 进化 |
Cloning and Sequence Analysis of Full-length cDNA of Actin Gene from Birch(Betula platyphylla Suk.) |
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| CHEN Peng-Fei LIU Xue-Mei, SONG Fu-Nan SONG Xing-Shun, LIU Ni JIN Wei-Wei LIU Wei.Cloning and Sequence Analysis of Full-length cDNA of Actin Gene from Birch(Betula platyphylla Suk.)[J].Bulletin of Botanical Research,2009,29(3):339-345. |
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| Authors: | CHEN Peng-Fei LIU Xue-Mei SONG Fu-Nan SONG Xing-Shun LIU Ni JIN Wei-Wei LIU Wei |
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| Institution: | 1.College of Life Science;Northeast Forestry University;Harbin 150040;2.Key Laboratory of Forest Tree Genetic Improvement and Biotechnology;Ministry of Education;Harbin 150040 |
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| Abstract: | Total RNA was isolated from Betula platyphylla xylem by the method of modified CTAB which was more effective and simple.The primers of plant actins conserved coding region were designed and a full-length cDNA of actin was obtained using RT-PCR and RACE techniques.Its full length is 1 785 bp.The sequence analysis result revealed that it contained a coding region of 1 134 bp encoding a protein of 378 amino acids,a 5'-UTR of 157 bp and a 3'-UTR of 495 bp.Homologous alignment shows that it shares over 80% nucle... |
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| Keywords: | Betula platyphylla Suk actin gene gene clone sequence analysis evolution |
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