蓝莓果渣提取物总酚含量及抗氧化活性研究

研究了蓝莓果渣提取物总酚含量及其抗氧化活性。分别采用水、40%乙醇及纤维素酶辅助乙醇超声提取蓝莓果渣,并用Folin-Ciocalteu试剂对3种提取物的总酚含量进行评估;并采用DPPH清除实验及O2—.清除实验对3种提取物的抗氧化活性进行研究。实验结果表明,纤维素酶辅助超声提取蓝莓果渣的总酚含量最高,可达425.36±15.21 mg GAE.100 g-1DW,远远高于水提物(169.46±9.75 mg GAE.100 g-1DW)及醇提物(218.39±12.54mg GAE.100 g-1DW)中的总酚含量。且纤维素酶辅助乙醇超声提取物对DPPH的清除能力为2.67±0.13 gVc.100 g-1DW,对O—.2的清除能力2.48±0.14 g Vc.100 g-1DW,明显好于醇提物及水提物抗氧化活性。

Total Phenolic Content and Antioxidant Activities of Blueberry Pomace Extracts

Total phenolics and antioxidant activities of blueberry pomace extracts were studied in the present study. Water, 40% ethanol and cellulase-ultrasonic-assisted extraction were used to extract blueberry pomace in this study. Folin-Ciocalteu method was employed for total phenol contents determination. Moreover, the antioxidant activities of aqueous extracts, ethanol extracts and cellulase-ultrasonic-assisted extracts were determined by DPPH free radical scavenging assay and O^-·₂ free radical scavenging assay. Results showed that total phenolic content of cellulase-ultrasonic-assisted extracts (425.36±15.2 mg GAE·100 g^-1 DW) was much higher than that of aqueous extracts (169.46±9.7 mg GAE·100 g^-1 DW) and ethanol extracts (218.39±12.5 mg GAE·100 g^-1 DW). The antioxidant activity of cellulase-ultrasonic-assisted extracts in the DPPH radical scavenging and superoxide radical scavenging assay were 2.67±0.13 g Vc·100 g^-1 DW and 2.48±0.14 g Vc·100 g^-1 DW, respectively. These values were much lower than those of the aqueous extracts and ethanol extracts.