小麦T型细胞质雄性不育系与可育系叶片蛋白质双向电泳分析

利用育性恢复基因(Rf3)的近等基因系1031-1、S-165和1031-1与S-165之间的正交与反交,创建了四个实验品系(1031-1、S-165、不育品系、反交品系);采用改进的蛋白质聚丙烯酰胺凝胶双向电泳技术,从发育遗传学的角度,对小麦T型细胞质雄性不育和可育株旗叶表达的相关蛋白产物进行差异分析。通过对旗叶蛋白的双向电泳分析,发现4个品系有相近的蛋白质双向电泳图谱。没有相应穗中差异蛋白质的出现。从蛋白质水平上证实了不育基因与恢复基因表达具有器官特异性特征。

Analysis of leaf proteins of T-cytoplasmic male sterility and fertility restoration in common wheat by 2D-PAGE

To explore the mechanisms underlying T-CMS(cytoplasmis male sterility). Four experimental lines of common wheat were studied. Two of them were the members of near isogenic lines of restorer, Rf3, namely T.aestivum cv.s165(B:rf3/rf3) and 1031-1(T:Rf3/Rf3). The other two lines were reciprocal hybrids between S165 and 1031-1, named CMS-line(T:rf3/rf3) and RC-line(B:Rf3/rf3). From the developmental genetics angle, the protein comparisons of T-cytoplasmic male sterility (CMS) and fertility restoration in common wheat are carried on by means of IEF-SDS PAGE with an improved silver stain technique. The four experimental lines have the same patterns of the proteins two -dimensional electrophoresis at the post-meiosis of microspores stages, which indicates the expression of the male sterility and its fertility restoration genes has organ-special characters.