| 花楸腋芽增殖途径快繁技术研究 |
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| 引用本文： | 郭艳茹,詹亚光,纪丽丽.花楸腋芽增殖途径快繁技术研究[J].植物研究,2007,27(2):224-228. |
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| 作者姓名： | 郭艳茹 詹亚光 纪丽丽 |
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| 作者单位： | 运城学院生命科学系 运城044000(郭艳茹)，东北林业大学生命科学学院 哈尔滨150040(詹亚光)，黑龙江林业职业技术学院 牡丹江157011(纪丽丽) |
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| 基金项目： | 大庆市科学技术计划项目 |
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| 摘 要： | 选用花楸茎节为外植体进行组培腋芽增殖途径研究，结果表明：利于花楸生长的最适宜基本培养基为MS培养基；芽诱导培养基为1/2MS+6-BA 2.5 mg·L-1+2.4-D 0.5 mg·L-1；增殖培养基为MS+6-BA（1.600~2.100）mg·L-1+NAA（0.140~0.230） mg·L-1，平均繁殖系数达到13倍以上；继代壮苗培养的最佳培养基为MS+6-BA 0.2 mg·L-1+IBA 0.2 mg·L-1；在1/2MS+IBA 0.3 mg·L-1培养基上，平均生根数为5.96，生根率达90.20%。将生根后的组培苗移栽至腐殖土：泥炭土：河沙（比例为3：2：1）的基质中，成活率达95.55%。
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| 关 键 词： | 花楸腋芽 组织培养 快速繁殖 |
| 修稿时间： | 2006-08-31 |
The Rapid Propagation Technology of Sorbus pohuashanensis by Axillary Bud Regeneration Way |
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| GUO Yan-Ru,ZHAN Ya-Guang,JI Li-Li.The Rapid Propagation Technology of Sorbus pohuashanensis by Axillary Bud Regeneration Way[J].Bulletin of Botanical Research,2007,27(2):224-228. |
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| Authors: | GUO Yan-Ru ZHAN Ya-Guang JI Li-Li |
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| Institution: | 1.Department of Life Science,Yuncheng University,Yuncheng 044000 2.College of Life Science, Northeast Forestry University,Harbin 1500403.Heilongjiang Forestry Vocation-Technical College,Mudanjiang 157011 |
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| Abstract: | In order to study the rapid propagation technology for Sorbus pohuashanensis stem segments with axillary buds were chosen as explants. The results showed that the best basic media was MS; axillary buds were induced successfully on MS basal media supplemented with 6-BA(2.5 mg·L-1) and 2.4-D(0.5 mg·L-1); the optimum medium for bud proliferation was MS+6-BA(1 600~2 100)mg·L-1+NAA(0.140~0.230)mg·L-1, 13 shoots per explant could be obtained at average;the shoots grew strongly on MS+6-BA0.2 mg·L-1+IBA0.2 mg·L-1; And 1/2MS+IBA0.3 mg·L-1 was the best medium for shoot rooting,with the rooting rate at 90.20%,and the average root number of 5.96;Plantlets survival rate reached 95.55% when transplanted on the mixture(humus 3:peat soil 2:sand 1). |
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| Keywords: | Sorbus pohuashanensis axillary bud tissue culture rapid propagation |
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