| 大孔树脂纯化卫矛总黄酮的工艺研究 |
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| 引用本文： | 赵占义,孙学斌,陈明刚,吴延丽,林锋,董陆陆.大孔树脂纯化卫矛总黄酮的工艺研究[J].植物研究,2009,29(1):124-128. |
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| 作者姓名： | 赵占义 孙学斌 陈明刚 吴延丽 林锋 董陆陆 |
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| 作者单位： | 1.哈尔滨医科大学药学院，哈尔滨 150086 ;2.远达药业集团博士后科研工作站，哈尔滨 150086 |
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| 基金项目： | 黑龙江省科技攻关项目(GC04C50103) |
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| 摘 要： | 筛选纯化卫矛总黄酮的最佳大孔树脂及其最佳工艺条件，采用正交设计法考察树脂种类、洗脱液浓度、pH值等因素对纯化的影响。用紫外分光光度法测定总黄酮的含量，计算吸附量、解吸率和浸膏总黄酮含量，最后确定最佳工艺条件。AB-8树脂最适于纯化卫矛总黄酮；最佳吸附量和解吸率分别是7.32 mg·g-1和90.98%；浸膏的总黄酮含量从7.64%提高到了52.12%。该树脂可以用于精制卫矛总黄酮，提高提取物中总黄酮的含量。
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| 关 键 词： | 卫矛 大孔树脂 正交法 纯化 |
Study on Purification Process of Total Flavonoids from Euonymus alatus(Thuhb) Sieb. by Macroporous Resins |
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| ZHAO Zhan-Yi SUN Xue-Bin CHEN Ming-Gang WU Yan-Li LIN Feng DONG Lu-Lu.Study on Purification Process of Total Flavonoids from Euonymus alatus(Thuhb) Sieb. by Macroporous Resins[J].Bulletin of Botanical Research,2009,29(1):124-128. |
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| Authors: | ZHAO Zhan-Yi SUN Xue-Bin CHEN Ming-Gang WU Yan-Li LIN Feng DONG Lu-Lu |
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| Institution: | 1.Pharmacy College of Harbin Medical University;Harbin 150086;2.Post-Doctor Working Station of Yuanda Group;Harbin 150086 |
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| Abstract: | The optimum macroporous resin and process were optimized for the purification of total flavonoids from Euonymus alatus,orthogonal-test method was adopted to study the effects of several factors including the kinds of resins,the concentration of eluent,the pH,etc.,on the purification.The concentration of flavonoids in E.alatus was determined by ultraviolet spectrophotometry,the adsorption capacity,the rate of desorption and the content of total flavonoids in the extract were calculated to determine the suita... |
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| Keywords: | Euonymus alatus macroporous resin orthogonal-test purification |
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