转衣藻染色质烟草细胞核的变化及衣藻核基因的表达

应用反复冻融法以衣藻原生质体为材料制备衣藻染色质,应用显微操作技术准确将衣藻染色质转移到烟草叶片外植体中,进行连续培养并镜检观察。结果表明,经染色质转移处理的烟草叶片外植体、衣藻细胞核与烟草细胞核均发生形态上的变化。同时烟草叶片外植体正常发芽并获得再生苗,经RT-PCR检测发现,有衣藻核基因(rbcs2)的表达。

The nuclear change of tobacco transformed with chromatin of Chlamydomonas reinhardtii and the expression of nuclear gene

Through the method of being frozen in the liquid nitrogen for a short time,and then thawing in the room temperature for several times,the protoplasts of Chlamydomonas reinhardtii were broken and chromatin were obtained.Then we exactly transformed the chromatin to the explants of the tobacco leaves by the micromanipulation,and then we cultured the explants,observed by the fluorescent microscope every day.The result indicated that the chromatin transformed explants,the nuclei of C.reinhardtii and tobacco were all changed.At the same time,the explants formed the callus and then grew into gemmule.Through the RT-PCR detection,we found the expression of the nuclear gene rbcs2.