| 欧美杨108号转蜘蛛杀虫肽与Bt毒蛋白嵌合基因的研究 |
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| 引用本文： | 遇文婧,邹传山,王志英,张国财.欧美杨108号转蜘蛛杀虫肽与Bt毒蛋白嵌合基因的研究[J].植物研究,2009,29(5):603-606. |
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| 作者姓名： | 遇文婧 邹传山 王志英 张国财 |
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| 作者单位： | (东北林业大学林学院，哈尔滨150040) |
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| 基金项目： | 黑龙江省攻关项目(GB06B303-4);;黑龙江省重点基金项目(ZJN04-0101);;东北林业大学校基金项目资助(07044) |
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| 摘 要： | 以欧美杨108号为外植体材料，利用农杆菌介导法成功地将抗虫基因（蜘蛛杀虫肽+Bt毒蛋白的嵌合基因）导入受体中。通过PCR检测，凝胶电泳结果显示8个卡那霉素抗性芽中，6个存在目的基因；经PCR-Southern印迹杂交检测，阳性率为50%，进一步表明目的基因成功整合到欧美杨108号基因组内。
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| 关 键 词： | 欧美杨108号 蜘蛛杀虫肽+Bt毒蛋白基因 PCR-Southern印迹杂交 |
Populus nigra×Populus deltoids “108” Transformed the Chimeric Genes of Spider Insecticidal and Bt |
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| YU Wen-Jing ZOU Chuan-Shan WANG Zhi-Ying ZHANG Guo-Cai.Populus nigra×Populus deltoids “108” Transformed the Chimeric Genes of Spider Insecticidal and Bt[J].Bulletin of Botanical Research,2009,29(5):603-606. |
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| Authors: | YU Wen-Jing ZOU Chuan-Shan WANG Zhi-Ying ZHANG Guo-Cai |
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| Institution: | College of Forestry;Northeast Forestry University;Harbin 150040 |
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| Abstract: | Using the agrobacterium-mediated approach, the anti-insect gene (the chimeric genes of spider insecticidal and Bt) was successfully transformed into the acceptor of Populus nigra×Populus deltoids “108” as explant. Through PCR analysis, the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes; Through PCR Southern blots analysis, the result showed that the positive rate was 50%, and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids “108” plants successfully. |
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| Keywords: | Populus nigra×Populus deltoids “108” the chimeric genes of spider insecticidal and Bt PCR-Southern blots analysis |
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