超临界CO2萃取红景天中红景天苷、苷元酪醇的研究

采用超临界CO2萃取法和乙醇常温浸提法相比较,研究从红景天中提取红景天苷、苷元酪醇的工艺条件,结论是:采用超临界CO2萃取法能萃取出红景天生药中红景天苷的1.2%,提取率不高,但该方法能萃取出80%的苷元酪醇,萃取液中苷元酪醇的相对含量可达45.68%;乙醇常温浸提法能将红景天苷、苷元酪醇同时有效萃取,且得率较高,但是萃取液中两物质相对含量较低,进一步分离纯化将有难度.本研究结果表明,将超临界CO2萃取法和乙醇常温浸提法有效结合,可实现两物质的有效分离,推进红景天有效成分的产业化进程.

Study on supercritical CO2 extraction of salidroside and P-tyrosol in Rhodiola sachalinensis

The process conditions for the extraction of salidroside and p-tyrosol from Rhodiola sachalinensis A Bor. was studied by using the comparison of supercritical CO 2 extraction method and ethanol extraction method at normal temperature. The conclusion was as following: the salidroside in Rhodiola sachalinensis raw materials could not be extracted effectively by supercritical CO 2 extraction method, which could only extract 1.2%of the salidroside but could extract 80%of the p-tyrosol in Rhodiola sachalinensis raw materials and the relative content of p-tyrosol in extracting solution could reach 45.68%; the salidroside and p-tyrosol could be extracted effectively at the same time by ethanol extraction method at normal temperature, however, the relative content of both materials in extracting solution was low and it would be difficult for further separation and purification. Our results indicated that effective separation will be realized and industrialization process of active components in Rhodiola sachalinensis will be pushed forward if supercritical CO 2 extraction method and ethanol extraction method at normal temperature are combined effectively.