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| 佛手山药组织培养的研究 | |
| 引用本文： | 刘金英,徐有明,李双来,郭春凤,郭治成.佛手山药组织培养的研究[J].植物研究,2006,26(3):323-328. |
| 作者姓名： | 刘金英 徐有明 李双来 郭春凤 郭治成 |
| 作者单位： | 1. 华中农业大学园艺林学学院,武汉,430070 2. 湖北省农科院,武汉,430064 3. 武穴市农业局,武穴,435400 |
| 基金项目： | 华中农业大学科技创新基金项目(049011) |
| 摘 要： | 以佛手山药块茎、叶片、茎段为外植体, 探讨了其组织培养技术。结果表明：块茎培养以暗培养MS+6-BA1.0 mg·L-1+NAA0 1 mg·L-1效果较好；叶片诱导的适宜培养基为MS+ 6-BA0.5～1.0 mg·L-1+NAA2.0 mg·L-1, 暗培养；茎段培养都是光培养，无节茎段以MS+6-BA1.0 mg·L-1+NAA0.5 mg·L-1较好；带节茎段的初代培养则以MS+6-BA0.5～1.0 mg·L-1+NAA0.1 mg·L-1效果较好，继代增殖培养基为MS+6-BA0.5 mg·L-1+NAA0.1 mg·L-1，生根培养基为1/2MS +NAA0.5 mg·L-1。 |
| 关 键 词： | 佛手山药 块茎 叶片 茎段 组织培养 |
| 收稿时间： | 2005-08-17 |
| 修稿时间： | 2005年8月17日 |
Tissue culture of Dioscorea opposita "foshou" |
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| LIU Jin-Ying,XU You-Ming,LI Shuang-Lai,GUO Chun-Feng,GUO Zhi-Cheng.Tissue culture of Dioscorea opposita "foshou"[J].Bulletin of Botanical Research,2006,26(3):323-328. | |
| Authors: | LIU Jin-Ying XU You-Ming LI Shuang-Lai GUO Chun-Feng GUO Zhi-Cheng |
| Institution: | 1.Huazhong Agricultural University,Wuhan 430070 2.Hubei Agricultural Institute,Wuhan 430064 3.Agricultural bureau of Wuxue City,Wuxue 435400 |
| Abstract: | Tissue culture of Dioscorea opposita “foshou” was studied with explants such as tubers，leaves，stem segments. The results showed that the media of MS+6-BA1.0 mg·L-1+NAA0.1 mg·L-1 was for tubers and the media of MS+6-BA0.5~1.0 mg·L-1+NAA2.0 mg·L-1 was for leaves in the dark condition. The tissue culture of stems was conducted in light condition. The optimum media was MS + 6-BA1.0 mg·L-1 + NAA0.5 mg·L-1 for the stem without the node. MS+ 6-BA0.5~1.0 mg·L-1 + NAA0.1 mg·L-1 was suitable for primary culture of the stem with the node, the optimum propagating media was MS+6-BA0.5 mg·L-1+ NAA0.1 mg·L-1, roots were induced on the media of 1/2MS+NAA0.5 mg·L-1. |
| Keywords: | Dioscorea opposita "foshou" tubers leaves stem segments tissue culture |
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