拟南芥叶片细胞包被系统酸性降解的光镜观察

观察了拟南芥叶片细胞包括细胞壁和质膜在内的细胞包被系统在酸性条件下酶促降解的过程。观察发现,处于酸性酶解液中的拟南芥叶片,最初细胞壁完整,细胞排列有序,其后细胞壁开始部分降解,细胞排列逐渐进入无序状态,随后细胞壁完全降解,去壁的原生质体完全进入游离状态,游离原生质体的质膜也随之降解,细胞器溢出后以细胞核为核心积聚、重组为新的原生质体。进一步观察了这一过程中细胞pH值的改变,结果发现,酸性酶解过程中细胞倾向于pH值降低,而细胞器重组产生的新原生质体pH值向正常水平恢复。因此,酸性环境对拟南芥叶片细胞包被系统的降解产生重要的影响。

Light microscopic observation of acid degradation on cell coat system of Arabidopsis thaliana leaves

This article mainly focuses on the degradation course of enzymatic reaction of cell coat system including cell wall and cytoplasma membrane of the Arabidopsis thaliana leaves under acid conditions. Under light microscopic observation, the cell walls of the leaves of A. thaliana which is under the acid condition are intact and arranged in order at first, thereafter the cell wall begins to degrade partly, and the cells tend to disordered state gradually, afterwards the cell walls are totally degraded. Protoplasts isolated by enzyme digestion totally enter the free state, and accompanied by the degradation of the cytoplasma membrane of the free protoplasts, having overflowed, the organelle surround the nucleus, and thus a new protoplast is reconstituted. We further observed the change of pH in the cell in this course. The result indicated that the cell tended to the pH value decrease during the course of the acid enzymatic degradation, and the pH of the protoplast reconstituted from the organelle came back to its normal level. In conclusion, the acid environment had an important effect on the degradation of the cell coat system of A. thaliana leaves.