| 濒危植物珙桐的组织培养与植株再生 |
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| 引用本文： | 邹利娟,苏智先,胡进耀,吴庆贵,苏瑞军,余阿梅.濒危植物珙桐的组织培养与植株再生[J].植物研究,2009,29(2):187-192. |
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| 作者姓名： | 邹利娟 苏智先 胡进耀 吴庆贵 苏瑞军 余阿梅 |
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| 作者单位： | (1.西华师范大学环境科学与生物多样性保护省级重点实验室，南充637002) ;(2.四川省生态与环境技术重点实验室，绵阳 621000) |
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| 基金项目： | 国家自然科学基金资助项目(30670209);;四川省科技厅应用基础项目(05JY029-107);;四川省教育厅重大培育项目(07ZZ015) |
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| 摘 要： | 以珙桐冬芽为材料进行组织培养和植株再生研究，结果表明：珙桐冬芽直接诱导丛生芽的最适培养基为WPM+NAA 0.2 mg·L-1+6-BA 3.0 mg·L-1+AC 2.0 g·L-1；珙桐带芽茎段增殖的适宜培养基为WPM+NAA 0.05 mg·L-1+6-BA 1.0 mg·L-1+GA3 2.0 mg·L-1+AC 2.0 g·L-1；生根最佳培养基为White+IBA3.0 mg·L-1+6-BA 1.0 mg·L-1+AC 2.0 g·L-1，在此条件下，根发育良好，植株健壮；组培苗炼苗后移栽，成活率可达80%。
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| 关 键 词： | 珙桐 冬芽 丛生芽 茎段增殖 植株再生 |
Tissue Culture and Plant Regeneration of Endangered Plant Davidia involucrate Baill |
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| ZOU Li-Juan, SU Zhi-Xian HU Jin-Yao WU Qing-Gui SU Rui-Jun YU A-Mei.Tissue Culture and Plant Regeneration of Endangered Plant Davidia involucrate Baill[J].Bulletin of Botanical Research,2009,29(2):187-192. |
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| Authors: | ZOU Li-Juan SU Zhi-Xian HU Jin-Yao WU Qing-Gui SU Rui-Jun YU A-Mei |
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| Institution: | 1.Sichuan Provincial Key Laboratory of Environmental Science and Biodiversity Conservation;China West Normal University;Nanchong 637002;2.Sichuan Provincial Key Laboratory of Ecology and Environmental Technology;Mianyang 621000 |
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| Abstract: | Using winter bud of Davidia involucrate as explant, we studied its tissue culture and plant regeneration. The results indicated: the best medium for the clump shoot induction directly from D. involucrata winter bud was WPM+NAA 0.2 mg·L-1+6-BA 3.0 mg·L-1+AC 2.0 g·L-1; the appropriate medium for shoot segment multiplying of D. involucrate bud was WPM+NAA 0.05 mg·L-1+6-BA 1.0 mg·L-1+GA3 2.0 mg·L-1+AC 2.0 g·L-1; the medium for rooting culture was White+IBA3.0 mg·L-1+6-BA 1.0 mg·L-1+AC 2.0 g·L-1, under these conditions, the roots were well developed and the plants were healthy; finally, about 80% of these seedlings could survival after transplantation. |
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| Keywords: | Davidia involucrate Baill winter bud clump shoot shoot segment multiplying plant regeneration |
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