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胡麻资源萌发期耐盐综合性评价
引用本文:赵玮,齐燕妮,张建平,赵利,王利民,谢亚萍,王斌,张艳萍.胡麻资源萌发期耐盐综合性评价[J].植物研究,2019,39(6):955-963.
作者姓名:赵玮  齐燕妮  张建平  赵利  王利民  谢亚萍  王斌  张艳萍
作者单位:1. 甘肃省农业科学院作物研究所, 兰州 730070;2. 甘肃省农业科学院生物技术研究所, 兰州 730070
基金项目:国家自然科学基金项目(31560347、31660368、31760426);兰州市人才创新创业项目(2017-RC-97);甘肃特色作物产业技术体系(胡麻岗位);甘肃省农业科学院科研条件建设及成果转化项目(2017GAAS22)
摘    要:筛选胡麻耐盐品种,对胡麻产业可持续发展应对盐渍胁迫具有重要现实意义。试验选用100 mmol·L-1的NaCl溶液对28份胡麻资源种子进行胁迫,并测定发芽率、总鲜重、下胚轴鲜重、下胚轴干重、下胚轴长、胚根鲜重、胚根干重和胚根长等相关指标,通过相关性分析、因子和主成分分析、聚类分析和逐步回归分析对11个指标进行综合分析,对28份胡麻资源萌发期耐盐性做出评价,并筛选出胡麻萌发期耐盐性鉴定的关键指标。结果表明:经相关分析,11个指标中多项指标间都是极显著或显著的正相关;因子和主成分分析将11个单项指标转化成3个综合指标,第Ⅰ主成分为下胚轴长势情况,第Ⅱ主成分为胚根长势情况,第Ⅲ主成分为种子萌发情况;聚类分析将28份胡麻资源划分为4大类:耐盐型、中等耐盐型、盐敏感型和强盐敏感型。雁杂10号、轮选1号为强耐盐材料;陇亚4号、R40号、DYMS为较强耐盐材料;逐步回归分析建立了胡麻萌发期耐盐能力预测的数学评价模型,筛选出总鲜重、胚根干重、发芽指数和下胚轴长这4个鉴定指标是胡麻萌发期耐盐能力鉴定的关键指标。以这4个筛选指标计算出的预测值与综合评价计算出的综合Z值之间极显著相关(R2=0.991 8**),表明鉴定结果具有较高的准确性,可以代替原来11个指标对胡麻萌发期耐盐性进行评价。

关 键 词:胡麻  萌发期  耐盐性  综合评价  指标筛选  
收稿时间:2019-05-31

Comprehensive Evaluation for Salinity Tolerance of Flax Resources During Germination
ZHAO Wei,QI Yan-Ni,ZHANG Jian-Ping,ZHAO Li,WANG Li-Min,XIE Ya-Ping,WANG Bin,ZHANG Yan-Ping.Comprehensive Evaluation for Salinity Tolerance of Flax Resources During Germination[J].Bulletin of Botanical Research,2019,39(6):955-963.
Authors:ZHAO Wei  QI Yan-Ni  ZHANG Jian-Ping  ZHAO Li  WANG Li-Min  XIE Ya-Ping  WANG Bin  ZHANG Yan-Ping
Institution:1. Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070;2. Institute of Biotechnology, Gansu Academy of Agricultural Science, Lanzhou 730070
Abstract:It has important practical significance for flax to screen salt tolerant varieties to deal with salt stress in the sustainable development In this study, the seed of 28 flax resources were treated with 100 mmol·L-1 NaCl and the germination rate, total fresh weight, hypocotyl fresh weight, hypocotyl dry weight, hypocotyl length, radicle fresh weight, radicle dry weight, radicle length were measured on the seventh day. Through correlation analysis, factor and principal components analysis, clustering analysis and stepwise regression analysis, the 11 indicators were comprehensively analyzed in order to comprehensively evaluate the salinity tolerance of 28 flax materials during germination, and screen out the key indicators for the salt-tolerance identification of flax materials during germination. The results showed that during the correlation analysis, there were extremely significant or significant and positive relationships among multiple indicators; the factor and principal components analysis turned 11 single indicators into 3 comprehensive indicators. The principal component Ⅰ mainly reflected the growth situation of hypocotyl; The principal component Ⅱ mainly reflected the growth situation of radicle; The principal component Ⅲ mainly reflected the germination situation of seed. The 28 flax materials were divided into 4 categories by clustering analysis:salt tolerant, medium salt tolerant, salt sensitive, and strongly salt sensitive. Among 28 flax materials, Yanza 10 and Lunxuan 1 were strongly salt tolerant; Longya 4、R40 and DYMS were salt tolerant. We built a mathematical evaluation model for predicting the salinity tolerance of flax resources during germination, then screened out 4 key indicators for salt-tolerance identification of flax resources during germination, including total fresh weight, radicle dry weight, germination index and hypocotyl length. The predictive values of 4 key indicators had a significantly correlation with the comprehensive Z values(R2=0.991 8**), which showed that the results were high accuracy, so the 4 key indicators could evaluate the salinity tolerance of flax resources during germination by taking place of 11 indicators.
Keywords:Flax  germination  salinity  comprehensive evaluation  indicators screening  
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