| 浙江省桐庐县长叶榧居群遗传结构的ISSR分析 |
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| 引用本文： | 李建辉；金则新.浙江省桐庐县长叶榧居群遗传结构的ISSR分析[J].植物研究,2011,31(6):722-728. |
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| 作者姓名： | 李建辉；金则新 |
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| 作者单位： | 1.华东师范大学环境科学系，上海 200062；;2.台州学院生态研究所，临海 317000 |
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| 基金项目： | 浙江省自然科学基金项目(Y5090258); 华东师范大学博士研究生科研创新项目(CX2011013) |
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| 摘 要： | 采用ISSR分子标记技术，对浙江省桐庐县白云源森林公园的6个长叶榧（Torreya jackii）亚居群进行分析，阐明长叶榧在小地理范围内的遗传结构。12个引物对6个长叶榧亚居群的120个个体进行扩增，共得到194个位点，其中多态位点87个，总多态位点百分率（P）为44.85%，平均为29.21%。长叶榧总Shannon信息指数（I）为0.166 3，平均为0.119 9；总Nei指数（h）为0.103 5，平均为0.075 5。P、I、h均表明长叶榧总体水平的遗传多样性较高，亚居群水平的遗传多样性较低。AMOVA分子变异分析显示，21.45%的变异存在于亚居群间，78.55%的变异存在于亚居群内。长叶榧亚居群间的遗传分化系数（Gst）为0.270 5，基因流为1.347 8。6个长叶榧亚居群的平均遗传距离为0.037 0，根据亚居群间的遗传距离进行UPGMA聚类，结果为生境相似的亚居群聚在一起。
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| 关 键 词： | 长叶榧 ISSR 亚居群 遗传多样性 遗传分化 |
ISSR Analysis of the Genetic Structure of Torreya jackii Population in Tonglu County,Zhejiang Province |
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| LI Jian-Hui, JIN Ze-Xin.ISSR Analysis of the Genetic Structure of Torreya jackii Population in Tonglu County,Zhejiang Province[J].Bulletin of Botanical Research,2011,31(6):722-728. |
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| Authors: | LI Jian-Hui JIN Ze-Xin |
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| Institution: | LI Jian-Hui1,2 JIN Ze-Xin2(1.Department of Environmental Science,East China Normal University,Shanghai 200062,2.Institute of Ecology,Taizhou University,Linhai 317000) |
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| Abstract: | Using inter-simple sequence repeat(ISSR) molecular technique,six subpopulations of Torreya jackii(Taxaceae) collected from Baiyunyuan Forest Park of Tonglu county of Zhejiang province were analyzed,the genetic structure of T.jackii was revealed in small scale.Using 12 primers,87 polymorphic loci from total 194 loci were detected in 120 individuals from 6 subpopulations and the overall percentage of polymorphic loci(P) was 44.85% with an average of 29.21%.Shannon's information index(I) and Nei's gene diversi... |
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| Keywords: | Torreya jackii ISSR subpopulation genetic diversity genetic differentiation |
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