耐冬山茶子叶再生体系的研究

青岛市市花——耐冬山茶为北方珍贵的冬季观赏物种,目前其栽培数量不能满足园林应用,亟需通过组培扩繁。以耐冬山茶的子叶为外植体,探讨不同因素对子叶愈伤组织诱导及植株再生的影响。结果表明,使用2%的NaClo对耐冬山茶果实灭菌的较佳时间是8 min,子叶愈伤组织的诱导率为100%。耐冬山茶子叶愈伤组织增殖的较优激素配比为0.5 mg·L^-1NAA+1.5 mg·L^-16-BA,其最大增殖量为4.06。将经过2次转接继代培养后的愈伤组织转入不定芽分化培养基MS+1.0mg/LNAA+10.0 mg·L^-16-BA进行不定芽的分化诱导,不定芽的分化率为10.7%。将耐冬山茶的不定芽芽条基部浸没在500 mg·L^-1的IBA溶液中70 min,再转接到1/2MS固体培养基,生根率达40%,成功建立了耐冬山茶子叶的植株再生体系。

Plant Regeneration System from Cotyledon of Camellia japonica(Naidong)

As the floral emblem of Qingdao,Camellia japonica(Naidong)is a precious ornamental speciesduring the winter of the north,but the quantity of C.japonica(Naidong)cultivars cannot meet requirements forapplication in landscape at present,so it is necessary to enlarge propagation by the means of tissue culture.Inorder to establish the regeneration system of C.japonica(Naidong),different factors affected on callus inductionand plantlet regeneration were studied from mature cotyledons of C.japonica(Naidong).The optimum timing ofsterilization in C.japonica(Naidong)seeds was soaked for 8 min in the 2%Na Clo,callus induction rate was100%.The optimal hormone combination of cotyledon callus subculture was 0.5 mg·L^-1NAA+1.5 mg·L^-16-BA,the multiplication rate was 4.06.After 2-time subcultured,the callus was transferred to MS+1.0 mg·L^-1NAA+10.0 mg·L^-16-BA for differentiation of adventitious buds,the rate of adventitious bud differentiation was 10.7%.The shoot base was immersed in 500 mg·L^-1IBA solution for 70 min,then transferred to 1/2 MS,the rate ofrooting could reach 40%.Regeneration system from mature cotyledons of C.japonica(Naidong)by inducingcallus,adventitious shoots and roots was established,which would provide technical support for the vegetativeproduction and innovation of its germplasm resources.