油菜BnMKK2基因的克隆及表达分析

通过实验从陇油6号油菜中克隆得到了一种新的MAPK激酶基因BnMKK2基因的cDNA，全长1 344 bp,其中包括5′非翻译区（5′UTR）111 bp，3′非翻译区（3′UTR）165 bp，开放阅读框（ORF）长1 068 bp，编码355个氨基酸，该基因编码的蛋白质分子量为39.3 kDa，理论等电点为6.8。与拟南芥AtMKK2有很高的同源性，因此命名为BnMKK2(GenBank登录号:HQ848661)。该基因实时荧光定量PCR分析表明，BnMKK2基因的表达受低温胁迫诱导。

Cloning and Real-time Expression Analysis of a Novel MAPK Kinase Gene nMKK2 in Brassica napus

A new MAPKK cDNA BnMKK2 was isolated from Brassica napus Longyou 6.The cloned full-length cDNA was 1 344 bp,contained a 5′ untranslated region(5′UTR) of 111 bp,a 3′ untranslated region(3′UTR) of 165 bp,and a 1 068 bp ORF.BnMKK2 encoded a protein of 355 amino acids with a deduced molecular weight of about 39.3k Da and with a PI of 6.8.The BnMKK2 exhibited closest homology to the Arabidopsis thaliana AtMKK2,so it was named BnMKK2(GenBank accession:HQ848661).Sequence Analysis indicated that the BnMKK2 protein exhibited closest homology to MAPKK,which is responsed to cold stress,and BnMKK2 played an important role in defending cold stress.Analysis by real-time RT-PCR indicated that the expression of BnMKK2 was increased in response to cold stress.