| 云南松外生菌根真菌分离培养研究 |
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| 引用本文： | 于富强,纪大干,刘培贵.云南松外生菌根真菌分离培养研究[J].植物研究,2003,23(1):66-71. |
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| 作者姓名： | 于富强 纪大干 刘培贵 |
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| 作者单位： | 中国科学院昆明植物研究所, 昆明 650204 |
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| 基金项目： | 国家自然科学基金 (No 30 0 70 0 0 4)，云南省自然科学基金 (No 2 0 0 0C0 0 6 8M ) |
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| 摘 要： | 就滇中一带云南松林进行了多次调查,共采集、鉴定标本130余号;从中选取13科, 19属, 33种真菌进行了菌种分离、培养的研究。选用并修改4种培养基进行了外生菌根真菌分离、培养的比较,结果共有11科, 17属, 25种真菌分离获得成功,成功率高达75.8%, 4种培养基的分离成功率依次为36.4%、63.6%、33.3%和57.6%。其中,修改PDA培养基和松针、玉米培养基较适宜于云南松外生菌根真菌的分离、纯化和培养。
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| 关 键 词： | 云南松 外生菌根真菌 纯培养 |
| 收稿时间： | 2002-08-16 |
| 修稿时间： | 2002年8月16日 |
STUDIES ON PURE CULTURE OF THE ECTOMYCORRHIZAL FUNGI ASSOCIATED WITH PINUS YUNNANENSIS |
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| YU Fu-Qiang,JI Da-Gan,LIU Pei-Gui.STUDIES ON PURE CULTURE OF THE ECTOMYCORRHIZAL FUNGI ASSOCIATED WITH PINUS YUNNANENSIS[J].Bulletin of Botanical Research,2003,23(1):66-71. |
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| Authors: | YU Fu-Qiang JI Da-Gan LIU Pei-Gui |
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| Institution: | Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204 |
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| Abstract: | Several surveys were carried out under Pinus yunnanensis Franch forest in Central Yunnan; the total collections are over 130. 33 species, belonging to 19 genera, 13 families were selected to supply a diversity of pure cultures. 4 types of media were selected for the pure culture study of ectomycorrhizal fungi. As a result, pure cultures of 25 species, 17 genera, and 11 families were obtained. The total isolation success rate reached 75.8%; the success rate of 4 media (No.1, No2, No.3, No.4) was 36.4%, 63.6%, 33.3% and 57.6% respectively. Modified PDA medium (No.2) and pine needles medium (No.4) are more suitable for the isolation, purification and maintenance of ectomycorrhizal fungi associated with P. yunnanensis. |
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| Keywords: | Pinus yunnanensis Franch ectomycorrhizal fungi pure culture |
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