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Genome-wide SNP identification by high-throughput sequencing and selective mapping allows sequence assembly positioning using a framework genetic linkage map |
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| Authors: | Jean-Marc Celton Alan Christoffels Daniel J Sargent Xiangming Xu D Jasper G Rees |
| Institution: | (1) Biotechnology Department, University of the Western Cape, Private Bag X17, 7535 Bellville, South Africa;(2) South African National Bioinformatics Institute, University of the Western Cape, Private Bag X17, 7535 Bellville, South Africa;(3) East Malling Research, New Road, ME19 6BJ East Malling, Kent, UK;(4) Agricultural Research Council, Biotechnology Platform, Private Bag X5, 0110 Onderstepoort, South Africa |
| Abstract: | BackgroundDetermining the position and order of contigs and scaffolds from a genome assembly within an organism's genome remains a technical challenge in a majority of sequencing projects. In order to exploit contemporary technologies for DNA sequencing, we developed a strategy for whole genome single nucleotide polymorphism sequencing allowing the positioning of sequence contigs onto a linkage map using the bin mapping method. |
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