Induction of protein body formation in plant leaves by elastin-like polypeptide fusions |
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| Authors: | Andrew J Conley Jussi J Joensuu Rima Menassa Jim E Brandle |
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| Institution: | (1) Department of Biology, University of Western Ontario, London, ON, Canada;(2) Southern Crop Protection and Food Research Centre, Agriculture and Agri- Food Canada, London, ON, Canada;(3) VTT Technical Research Centre of Finland, Espoo, Finland;(4) Vineland Research and Innovation Centre, Vineland Station, ON, Canada |
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| Abstract: | Background Elastin-like polypeptides are synthetic biopolymers composed of a repeating pentapeptide 'VPGXG' sequence that are valuable
for the simple non-chromatographic purification of recombinant proteins. In addition, elastin-like polypeptide fusions have
been shown to enhance the accumulation of a range of different recombinant proteins in plants, thus addressing the major limitation
of plant-based expression systems, which is a low production yield. This study's main objectives were to determine the general
utility of elastin-like polypeptide protein fusions in various intracellular compartments and to elucidate elastin-like polypeptide's
mechanism of action for increasing recombinant protein accumulation in the endoplasmic reticulum of plants. |
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