Generation of a tamoxifen inducible Tnnt2MerCreMer knock‐in mouse model for cardiac studies |
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| Authors: | Jianyun Yan Nishat Sultana Lu Zhang David S Park Akshay Shekhar Jun Hu Lei Bu Chen‐Leng Cai |
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| Institution: | 1. Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and the Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, New York;2. Leon H. Charney Division of Cardiology, New York University School of Medicine, New York, New York |
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| Abstract: | Tnnt2, encoding thin‐filament sarcomeric protein cardiac troponin T, plays critical roles in heart development and function in mammals. To develop an inducible genetic deletion strategy in myocardial cells, we generated a new Tnnt2:MerCreMer (Tnnt2MerCreMer/+) knock‐in mouse. Rosa26 reporter lines were used to examine the specificity and efficiency of the inducible Cre recombinase. We found that Cre was specifically and robustly expressed in the cardiomyocytes at embryonic and adult stages following tamoxifen induction. The knock‐in allele on Tnnt2 locus does not impact cardiac function. These results suggest that this new Tnnt2MerCreMer/+ mouse could be applied towards the temporal genetic deletion of genes of interests in cardiomyocytes with Cre‐LoxP technology. The Tnnt2MerCreMer/+ mouse model also provides a useful tool to trace myocardial lineage during development and repair after cardiac injury. genesis 53:377–386, 2015. © 2015 Wiley Periodicals, Inc. |
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| Keywords: | Tnnt2 MerCreMer cardiomyocyte tamoxifen heart |
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