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| 褪黑素对谷氨酸钠致痫大鼠脑内一氧化氮含量的影响 | |
| 引用本文： | 卢敏,王从荣,周厚纶.褪黑素对谷氨酸钠致痫大鼠脑内一氧化氮含量的影响[J].中国组织化学与细胞化学杂志,2009,18(1). |
| 作者姓名： | 卢敏 王从荣 周厚纶 |
| 作者单位： | 1. 河南科技大学医学院,解剖学系,洛阳,471003 2. 嘉兴市嘉兴学院梁林校区医学院,解剖学系,嘉兴,314001 3. 华中科技大学同济医学院,解剖学系,武汉,430030 |
| 基金项目： | 华中科技大学科学研究基金 |
| 摘 要： | 目的观察褪黑素(Melatonin,MT)对谷氨酸钠致痫大鼠脑内一氧化氮(nitric oxide,NO)含量的影响,研究其抑制癫痫的作用机制。方法40只健康雄性SD大鼠随机分为4组(每组10只):生理盐水对照组(NS组);谷氨酸钠致痫组(Glu组);褪黑素+谷氨酸钠组(MT+Glu组);Luzidole+褪黑素+谷氨酸组(Luz+MT+Glu组)。观察大鼠行为变化,记录脑电图,用NADPH组织化学反应检测大鼠海马内NO含量变化。结果行为学观察和EEG显示,NS组无痫样发作和痫样放电,Glu组和Luz+MT+Glu组痫样发作重(Ⅲ-Ⅴ级),脑电图显示频发高幅的痫样波,MT+Glu组有轻微发作(0-Ⅱ级),脑电图上偶见散在单个微小痫样波;NADPH组织化学反应结果显示,Glu组和Luz+MT+Glu组大鼠大脑皮质及海马内NOS阳性细胞与对照组比较增多,差异性明显(P<0.05),MT+Glu组较Glu组和Luz+MT+Glu组内NOS阳性细胞减少,差异性明显(P<0.05)。结论MT对谷氨酸钠致痫大鼠痫样发作程度、痫样放电有抑制作用,其机制之一是经其特异性受体,减弱NO作用,进而发挥抑痫效应。 |
| 关 键 词： | 癫痫 一氧化氮 褪黑素 谷氨酸钠 海马 皮质 |
EFFECT OF MELATONIN ON NO LEVEL IN BRAIN OF RATS WITH SEIZURES INDUCED BY GLUTAMATE |
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| Lu Min,Wang Congrong,Zhou Houlun.EFFECT OF MELATONIN ON NO LEVEL IN BRAIN OF RATS WITH SEIZURES INDUCED BY GLUTAMATE[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(1). | |
| Authors: | Lu Min Wang Congrong Zhou Houlun |
| Institution: | 1Deparment of Anatomy;Medical College;Henan University of Science and Technology;Luoyang;471003;2Deparment of Anatomy;Jiaxing Medical College;Jiaxing 314001;3Deparment of Anatomy;Tongji Medical College;Huazhong University of Science and Technology;Wuhan 430030;China |
| Abstract: | Objective To observe the effect of melatonin on NO level in the brain of rats with seizures induced by glutamate, and to explore the role of melatonin in epileptogenesis.Methods Forty male adult Sprague Dawley (SD) rats were randomly divided into four groups with 10 each: control group, L-glutamate group, melatonin intervening group and luzidole + melatonin + L-glutamate group. The behavior and electroencephalogram (EEG) of rats were observed and recorded. The level of NO in hippocampus was demonstrated wit... |
| Keywords: | Epilepsy Nitric oxide Melatonin L-glutamate Hippocampus Cortex |
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