非小细胞肺癌中细胞凋亡与P53、Bcl-2蛋白的表达及对预后的影响

目的研究细胞凋亡及凋亡相关蛋白P53和Bcl-2在非小细胞肺癌组织中的表达水平,及其对预后的影响。方法应用DNA缺口末端标记技术和免疫组化方法检测111例肺癌患者组织中细胞凋亡、突变型P53和Bcl-2蛋白表达水平。结果111例肺癌中,细胞凋亡高表达53例(47.7%),突变型P53蛋白阳性45例(40.5%),Bcl-2蛋白阳性59例(53.2%)。COX模型多因素分析显示,淋巴结的转移和Bcl-2蛋白的阳性表达是本组肺癌患者的预后不良因素(P<0.05)。结论凋亡及凋亡相关蛋白P53和Bcl-2影响肺癌患者的生物学行为及预后。

PROGNOSTIC SIGNIFICANCE OF EXPRESSIONS OF APOPTOSIS, P53 AND Bcl-2 IN NON-SMALL CELL LUNG CANCER

Objective To investigate the prognostic significance of the expressions of apoptosis and its correlating proteins P53 and Bcl-2 in non-small cell lung cancer.Methods The expression of apoptosis and the positive expression of P53 and Bcl-2 proteins were observed in 111 cases of lung cancer by using DNA nick end labeling(TUNEL) technique and immunohistochemical staining.Results The high expression of cell apoptosis and the positive rates ofmutatedP53 and Bcl-2 protein expressions in the specimens of 111 lung cancer were 53(47.7%),45(40.5%) and 59 cases(53.2%),respectirely.The COX model showed that the lymph node metastasis and the positive expression of Bcl-2 protein were adverse factors to the prognosis of lung cancer patients(P<0.05).Conclusion Apoptosis and the correlated proteins P53 and Bcl-2 are the factors that affect the biological behavior and prognosis of lung cancer.