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| 局灶性脑缺血后海马齿状回神经发生及其与血管内皮生长因子关系的研究 | |
| 引用本文： | 程海霞,张立平,胡坚莉,赵舒武,吴镇.局灶性脑缺血后海马齿状回神经发生及其与血管内皮生长因子关系的研究[J].中国组织化学与细胞化学杂志,2009,18(2):192-197. |
| 作者姓名： | 程海霞 张立平 胡坚莉 赵舒武 吴镇 |
| 作者单位： | 1. 天津市塘沽区中医院病理科,天津,300451 2. 山东大学医学院解剖学教研室,济南,250012 3. 天津中医药大学组织胚胎学教研室,天津,300193 4. 山东大学第二医院检验科,济南,250033 |
| 摘 要： | 目的研究成年大鼠局灶性脑缺血后海马齿状回（DG）神经发生的情况及其与血管内皮生长因子（VEGF）的关系,探讨脑缺血后神经发生及其调控机制。方法通过大脑中动脉阻断法（MCAO）建立大鼠局灶性脑缺血模型,以5-溴-2-脱氧尿核苷（BrdU）标记增殖的神经前体细胞（NPCs）,用免疫组化及免疫荧光双标记法动态检测脑缺血后不同时间DG神经细胞增殖及其分化,同时观察增殖细胞表达VEGF及其受体情况。结果与对照组相比,缺血侧DG的BrdU阳性细胞数在脑缺血后1d开始增加,7d达高峰,28d接近正常水平;BrdU/TuJ1、BrdU/MAP-2阳性双标细胞数在脑缺血后14d开始增加,28d达高峰;BrdU/GFAP阳性双标细胞数则无明显变化;增殖的BrdU阳性细胞同时表达VEGF及其受体FLK-1。结论大鼠局灶性脑缺血可激活DG自体NPCs原位增殖、分化,增殖的细胞同时表达VEGF及其受体可能是脑缺血后神经发生增强的调节机制之一。 |
| 关 键 词： | 局灶性脑缺血 海马齿状回 神经发生 血管内皮生长因子 细胞增殖 |
NEUROGENESIS IN DENTATE GYRUS OF THE HIPPOCAMPUS AND ITS CORRELATION WITH VASCULAR ENDOTHELIAL CELL GROWTH FACTOR AFTER FOCAL CEREBRAL ISCHEMIA |
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| Cheng Haixia,Zhang Liping,Hu Jianli,Zhao Shuwu,Wu Zhen.NEUROGENESIS IN DENTATE GYRUS OF THE HIPPOCAMPUS AND ITS CORRELATION WITH VASCULAR ENDOTHELIAL CELL GROWTH FACTOR AFTER FOCAL CEREBRAL ISCHEMIA[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(2):192-197. | |
| Authors: | Cheng Haixia Zhang Liping Hu Jianli Zhao Shuwu Wu Zhen |
| Institution: | 1Department of Pathology;Tianjin Tanggu Traditional Chinese Medical Hospital;Tianjin 300451;2Department of Anatomy;School of Medicine;Shandong University;Jinan 250012;3Department of Histologyand Embryology;Tianjin University of Traditional Chinese Medicine;Tianjin 300193;4Department of Laboratory Medicine;The Second Hospital of Shandong University;Jinan 250033;China |
| Abstract: | Objective To investigate neurogenesis and its relation with VEGF in the dentate gyrus(DG)of the hippocampus after focal cerebral ischemia of adult rats,and to discuss its regulatory mechanism.Methods Focal cerebral ischemia was induced by transient MCAO in adult Wistar rats.BrdU was injected to label dividing cells.Immunohistochemistry and immunofluorescence were used to detect the dynamic expression of BrdU,TuJ1,MAP-2 and GFAP which are the specific markers of endogenous neural precursor cells(NPCs),neuron... |
| Keywords: | Focal cerebral ischemia Dentate gyrus of the hippocampus Neurogenesis Vascular endothelial cell growth factor Cell proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |