| siRNA抑制Survivin基因对大肠癌细胞HCT116增殖及凋亡的的影响 |
| |
| 引用本文： | 曹立宇,丁丽红,尹玉,江燕,李昊,张洪福.siRNA抑制Survivin基因对大肠癌细胞HCT116增殖及凋亡的的影响[J].中国组织化学与细胞化学杂志,2010,19(2):168-171. |
| |
| 作者姓名： | 曹立宇 丁丽红 尹玉 江燕 李昊 张洪福 |
| |
| 作者单位： | 1. 安徽医科大学病理教研室,合肥,230032
2. 安徽医科大学病理教研室,合肥,230032;温州医学院第一附属医院病理科,浙江,325027 |
| |
| 基金项目： | 安徽高校省级自然科学研究课题 |
| |
| 摘 要： | 目的探讨si RNA沉默Survivin基因后对人大肠癌细胞系HCT116的Survivin蛋白表达、细胞增殖及凋亡的影响。方法以脂质体lip-2000为载体,用针对Survivin特异靶点的si RNA转染人大肠癌细胞系HCT116后,应用免疫细胞化学S-P法、Western Blot检测Survivin蛋白表达变化;MTT法检测细胞增殖;流式细胞技术检测细胞凋亡。结果免疫细胞化学结果显示:HCT116的正常对照组、脂质体对照组及阴性错配对照组细胞浆均呈强阳性表达,Survivin-si R-NA组细胞浆Survivin呈弱阳性表达;Western blot结果显示:HCT116细胞系Survivin-si RNA组细胞的蛋白条带亮度均明显低于正常对照组、脂质体对照组和阴性错配对照组;MTT检测结果:与阴性错配对照组相比,Survivin-si RNA组细胞生长出现明显的抑制(P0.05),不同时段(24h、48h、72h)肿瘤细胞增殖抑制率之间有显著差异(P0.05)。流式细胞术检测Survivin-si RNA组细胞凋亡比例为9.72%,明显高于空白对照组及阴性错配对照组(P0.01)。结论si RNA抑制Survivin基因可以抑制大肠癌细胞增殖,促进凋亡;Survivin有望成为大肠癌基因治疗的新靶点。
|
| 关 键 词： | 结直肠肿瘤 癌 Survivin RNA干扰 细胞凋亡 |
INFLUENCE OF siRNA-MEDIATED INHIBITING SURVIVIN GENE ON PROLIFERATION AND APOPTOSIS OF HCT116 CELLS |
| |
| Cao Liyu,Ding Lihong,Yin yu,Jiang yan,Li hao,Zhang Hongfu.INFLUENCE OF siRNA-MEDIATED INHIBITING SURVIVIN GENE ON PROLIFERATION AND APOPTOSIS OF HCT116 CELLS[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(2):168-171. |
| |
| Authors: | Cao Liyu Ding Lihong Yin yu Jiang yan Li hao Zhang Hongfu |
| |
| Institution: | Cao Liyu1,Ding Lihong1,2,Yin yu1,Jiang yan1,Li hao1,Zhang Hongfu 1 (1Department of Pathology,Anhui Medical Univerisity,Hefei 230032.2the First affiliated Hospital,Wenzhou Medical College,Zhejiang 325027,China) |
| |
| Abstract: | Objective To explore the expression of survivin protein and the effect of transfection of Survivin-siRNA on the proliferation and apoptosis of human colorectal cancer HCT116 cells.Methods Survivin-targeted siRNA was transfeted into human colorectal cancer HCT116 cells with lipofectamine 2000.Survivin protein expression was detected by immunocytochemistry and Western Blot.Cell proliferation was measured by MTT assay.Cell apoptosis was detected by flow cytometry.Results The HCT116 cells showed strongly positi... |
| |
| Keywords: | Survivin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
