胃癌survivin基因mRNA和蛋白的表达与临床病理关系

目的　研究胃癌组织中survivin基因的mRNA和蛋白表达情况及其与临床病理参数的关系。方法　应用原位杂交方法和免疫组化SP法检测 5 4例胃癌 ,34例胃良性病变 ,2 0例胃正常组织标本中survivin基因mRNA及蛋白的表达。并对其与临床病理因素和二者的关系进行分析。结果　Survivin蛋白表达阳性率在胃癌、良性疾病组织和正常组织分别为 87 0 % (47/ 5 4 )、 2 3 5 % (8/ 34)和 15 % (3/ 2 0 ) ;SurvivinmRNA阳性率为 79 6 % (43/ 5 4 )、 2 3 5 % (8/ 34)和2 0 % (4/ 2 0 )。胃癌组远大于正常胃组织和良性疾病组 ,而正常胃组织与胃良性疾病组之间差异无显著性。SurvivinmRNA与蛋白在胃癌中的表达呈正相关 (rs=0 6 79,P <0 0 5 )。且其阳性率高低与性别、年龄、组织学类型无关 ;而与淋巴结转移、TNM分期、组织学分级有关。结论　SurvivinmRNA与蛋白在胃癌中表达较高 ,且与淋巴结转移、TNM分期、组织学分级有关 ,它可作为评估胃癌生物学行为和判断预后的生物学指标。

RELATIONSHIP BETWEEN EXPRESSION OF SURVIVIN GENE mRNA AND PROTEIN IN HUMAN GASTRIC CARCINOMA AND CLINICAL PATHOLOGIC RELATION

? Objective To investigate the expression of survivin gene mRNA and protein in human gastric carcinoma tissue and its relation to clinical pathologic parameters.Method The mRNA and protein expression of survivingenewere examined in 54 malignant, 34 benign and 20 normal gastric lesions respectively by in situ hybridization (ISH) with transcriped antisense RNA probes and immunohistochemical method(SP technique). The relationship between the expression of survivin gene mRNA and protein in human gastric carcinomaand clinical pathologic parameters was analyzed. Result The positive rates of survivin protein in malignant, benign and normal gastric tissues were 87.0%(47/54), 23.5%(8/34)and 15%(3/20) and the positive rates of survivin mRNA were 79.6%(43/54), 23.5%(8/34) and 20%(4/20). The positive rate in malignant gastric lesions was significantly higher than that in the other two groups.The difference wasnotstatistically significant between benign and normal gastric tissues. A positive correlation was found between the expression of survivin mRNA and protein(r s0. 679,P<0.05). The positive rate in malignant gastric lesions was related with clinic pathological parameters including lymphnode metastasis, TNM staging and histological grade but not with sex, age and histological type. Conclusion The expression of survivin gene mRNA and protein was higher in human gastric carcinoma and was related with lymphnode metastasis, TNM staging and histological grade. It might be a biological index to evaluate the prognosis and biological behavior of gastric carcinoma.