造血干细胞动员与移植治疗脑梗死的对比研究

目的对比造血干细胞（HSCs）内源性动员与外源性移植治疗大鼠脑梗死疗效。方法根据Longa线栓法建立大鼠大脑中动脉闭塞模型（MCAO），7d后随机分成四组：A组（HSCs移植组）、B组（PBS组）、C组（HSCs动员组）、D组（对照组）。A组定向植入1×10^6个HSCs；B组植入PBS液；C组皮下注射重组人粒细胞集落刺激因子（rhG—CSF）10ug／kg／d，连用5d；D组不进行干预。移植前、后进行NSS评分；经免疫组化观察CD34、Nestin、VEGF、vWF阳性细胞的分布情况；TCC染色观察梗死体积的变化。结果第1周A组梗死侧半球CD34、Nestin、VEGF、vWF细胞高于其它3组（P〈0．01），C组Nestin、VEGF、vWF细胞高于B组和D组（P〈0．05）；第4周各组未见CD34细胞，A、C两组Nestin、VEGF细胞减少，vWF细胞增多（P〈0．05）；A组Nestin、VEGF、vWF细胞高于其它3组（P〈0．01），B、C、D组Nestin、VEGF细胞的差异无统计学意义，C组vWF细胞高于B组、D组（P〈0．05）。A组神经功能改善最显著，C组次之，B、D组间差异无统计学意义（P〉0．05）。A组梗死体积明显缩小（P〈0．01），B、C、D组间无差异（P〉0．05）。结论HSCs外源性移植对脑梗死后袄的大鼠治疗效果好于动员。

COMPARISON BETWEEN MOBILIZATION AND TRANSPLANTATION OF HEMATOPOIETIC STEM CELLS FOR THERAPY OF CEREBRAL INFARCTION IN RATS

Objective To Compare the effects of mobilization and transplantation of HSCs on cerebral infarction in rats. Methods MCAO models were done according to Longa＇s method and all adult male rats were randomly divided into 4 groups seven days later： group A （HSC transplantation）, group B （PBS）, group C （HSC mobilization）, group D （control）. We injected 1 × 10^6HSCs into the marginal zone of infarction in group A; and PBS in group B. Rats of group C were injected subcutaneously with rhG-CSF 20/lg/kg for the first time, and later 10ug/kg/d for 5 days;Group D did not receive any treatment. NSS tests were performed before and after treatment. Immunohistochemisty was used to observe the distribution of CD34,Nestin,VEGF and vWF cells in the ipsilateral hemisphere of the infarction.Infarction volume was measured by TTC staining. Results 1 week after treatment there were more CD34,Nestin,VEGF and vWF- positive cells in group A than in the other three groups （P〈0.01）;more Nestin,VEGF and vWF-positive cells in group C than in group B and D（P〈0.05）.At 4 weeks no CD34-positive cells were found in any group; The numbers of Nestin and VEGF-positive cells in group A and C were reduced remarkably compared with those at 1 week （P〈0.05）; In contrast,there was a significant increase in numbers of vWF cells （P〈0.05）. The quantities of Nestin, VEGF and vWF- positive cells in group A were statistically higher than those in the other three groups （P〈0.01）.There was no difference in numbers of Nestin and VEGF-positive cells between group B and D. vWF-positive cells in group C were more than those in group B and D（P〈0.05）. Most significant recovery of neurological function was in group A（P〈0.01）,secondly in group C, and there was no difference between group B and D.The infarction volume of group A was markedly decreased（P〈0.01）; no difference was found between groups B,C and D. Conclusion The therapeutic effect of transplantation on local cerebral infarction in rats is better than mobilization seven days after the onset ofinfartion.