| 人骨髓间充质干细胞分化为运动神经元过程中Nestin与Hb9表达的研究 |
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| 引用本文： | 张振山,王春芳,胡风云,李鹏飞,张海滨,李宵.人骨髓间充质干细胞分化为运动神经元过程中Nestin与Hb9表达的研究[J].中国组织化学与细胞化学杂志,2014(1):82-86. |
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| 作者姓名： | 张振山 王春芳 胡风云 李鹏飞 张海滨 李宵 |
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| 作者单位： | [1]山西医科大学实验动物中心,太原030001 [2]山西省人民医院,太原030001 [3]山西医科大学科研实验中心,太原030001 |
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| 基金项目： | 国家自然科学基金资助（81371384）;山西省实验动物专项资金资助（2011k06）;山西省实验动物专项资金资助（2012k01） |
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| 摘 要： | 目的探讨全反式维甲酸(RA)与音猬因子(Shh)诱导人骨髓间充质干细胞向神经干细胞乃至运动神经元方向分化过程中Nestin与Hb9的表达变化情况。方法采用传至第3代的人骨髓间充质干细胞,经含有RA与Shh的诱导液处理后,应用免疫细胞化学及荧光定量PCR方法分析Nestin与Hb9表达的变化情况。结果人骨髓间充质干细胞经过RA与Shh诱导后Nestin与Hb9的表达明显高于未加RA与Shh的对照组及空白组。结论 hBMSC具有向神经干细胞乃至运动神经元方向分化的潜能,RA与Shh可以在体外诱导hBMSC定向向该方向分化。
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| 关 键 词： | 骨髓间充质干细胞 运动神经元 Nestin Hb 人 |
Expression of Nestin and Hb9 during the differentiation of human bone marrow mesenchymal stem cells into Motor Neurons |
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| Zhang Zhenshan,Wang Chunfang,Hu Fengyun,Li Pengfei,Zhang Haibin,Li Xiao.Expression of Nestin and Hb9 during the differentiation of human bone marrow mesenchymal stem cells into Motor Neurons[J].Chinese Journal of Histochemistry and Cytochemistry,2014(1):82-86. |
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| Authors: | Zhang Zhenshan Wang Chunfang Hu Fengyun Li Pengfei Zhang Haibin Li Xiao |
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| Institution: | 1 Experimental Animal Center, Shanxi Medical University , Taiyuan 030001, China ; 2 Shanxi Provincial Peopge＇s Hospital, Taiyuan 030001, China; 3 Scientific Research and Experimental Center ,Shanxi Medical University, Taiyuan 030001, China) |
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| Abstract: | Objective To detect the expression changes of Nestin and Hb9 gene during the differenti-ation of human bone marrow mesenchymal stem cells into Motor Neurons. Methods The third generation of hBMSCs was used to induce differentiation of motor neurons by a limited medium containing RA and Shh. Then the real time quantitative PCR and immunofluorescence were used to detect the expression chan ges of Nestin and Hb9 gene. Results After RA and Shh induction, the expression of Nestin and Hb9 was significantly higher in hBMSC than in the two control groups without RA and Shh. Conclusion The hBM SCs have the potential ability to differentiate into neural stem cells and motor neurons. Shh and RA to gerber induce hBMSCs to differentiate into neural stem cells and motor neurons. |
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| Keywords: | Bone marrow mesenchymal stem cells Motor neuron Nestin Hb9 Human |
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