| FISH检测快速诊断曲霉菌感染的临床价值 |
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| 引用本文： | 孟浦,肖晶,潘峰,聂秀,周东风,何世斌,李立家.FISH检测快速诊断曲霉菌感染的临床价值[J].中国组织化学与细胞化学杂志,2010,19(4):331-334. |
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| 作者姓名： | 孟浦 肖晶 潘峰 聂秀 周东风 何世斌 李立家 |
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| 作者单位： | 1. 华中科技大学同济医学院附属协和医院儿科,武汉,430022
2. 武汉大学生命科学学院,武汉,430072 |
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| 摘 要： | 目的探讨荧光原位杂交技术(FISH)检测曲霉菌感染的可行性和特异性,以期早期病原学诊断临床真菌感染。方法采用地高辛末端标记的18S-1寡核苷酸探针与27例经HE及PAS染色诊断为可疑真菌感染的石蜡包埋组织切片进行FISH检测。结果 HE及PAS染色可疑真菌感染分别为23例和27例。27例可疑真菌感染标本14例检出FISH阳性信号。结果 FISH可以特异性地检测出组织石蜡切片中的曲霉菌,设计不同菌属探针进行靶DNA杂交可以快速检测各种临床标本中的真菌感染,从而作为IFI早期病原学诊断的一种新策略。
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| 关 键 词： | 曲霉菌 荧光原位杂交 寡核苷酸探针 侵袭性真菌感染 早期诊断 |
CLINICAL VALUE OF FISH IN THE DETECTION OF ASPERGILLUS INFECTION FOR RAPID DIAGNOSIS |
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| Meng Pu,Xiao Jing,Pan Feng,Nie Xiu,Zhou Dongfeng,He Shibin,Li Lijia.CLINICAL VALUE OF FISH IN THE DETECTION OF ASPERGILLUS INFECTION FOR RAPID DIAGNOSIS[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(4):331-334. |
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| Authors: | Meng Pu Xiao Jing Pan Feng Nie Xiu Zhou Dongfeng He Shibin Li Lijia |
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| Institution: | Meng Pu1,Xiao Jing1,Pan Feng1,Nie Xiu1,Zhou Dongfeng1,He Shibin2,Li Lijia2(1Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,2College of Life Science,Wuhan University,Wuhan 430072,China) |
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| Abstract: | Objective To evaluate the prospect of fluorescence in situ hybridization(FISH) technology in the diagnosis of pediatric invasive fungal infections.Methods 23-base pair oligonucleotides,which were complementary to 18S(18S-1 probes) rRNA of aspergillus and labeled with digoxigenin(DIG) were used as probes.27 formalin-fixed and paraffin-embedded surgical tissue specimens from patients with suspected fungal infection were included in this research.Then these tissue specimens were stained with HE and PAS simulta... |
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| Keywords: | Aspergillus Fluorescence in situ hybridization Oligonucleotide probes Invasive fungal infections Early diagnosis |
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