| 神经节苷脂GM1对帕金森模型小鼠黑质细胞凋亡及Bcl-2和Bax表达的影响 |
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| 引用本文： | 吴乐,刘子建,陈文军,黎红华.神经节苷脂GM1对帕金森模型小鼠黑质细胞凋亡及Bcl-2和Bax表达的影响[J].中国组织化学与细胞化学杂志,2007,16(4):474-477. |
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| 作者姓名： | 吴乐 刘子建 陈文军 黎红华 |
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| 作者单位： | 1. 广州军区武汉总医院神经内科,武汉,430070
2. 华中科技大学同济医学院解剖学系,武汉,430030 |
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| 摘 要： | 目的观察神经节苷脂GM1对帕金森模型小鼠黑质细胞凋亡及Bcl-2和Bax表达的影响,探讨其预防帕金森病的机制。方法成年C57-BL雄性小鼠分为正常对照组、模型组和神经节苷脂GMI治疗组。应用TUNEL法和免疫荧光组织化学染色技术观察各组小鼠黑质神经元凋亡及Bcl-2和Bax表达情况。结果TUNEL、Bcl-2和Bax阳性神经元主要位于黑质致密部。模型组黑质神经元凋亡数明显多于正常对照组,神经节苷脂GM1组黑质神经元凋亡数明显少于模型组。在模型组Bcl-2阳性神经元和Bax阳性神经元的数量均较正常对照组明显增多;在神经节苷脂GM1组Bcl-2阳性神经元的数量较模型组明显增多,但Bax阳性神经元的数量较模型组明显减少。结论神经节苷脂GM1可能通过增强黑质神经元内Bcl-2基因表达及抑制Bax基因表达的途径抑制黑质神经元的凋亡。
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| 关 键 词： | 神经节苷脂GM1 帕金森病 凋亡 Bax Bcl-2 |
| 修稿时间： | 2006-11-30 |
EFFECTS OF GANGLIOSIDE GM1 ON APOPTOSIS AND BCL-2 AND BAX EXPRESSIONS OF SUBSTANTIA NIGRA NEURONS IN MICE WITH PARKINSON'S DISEASE |
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| Wu Le,Liu Zijian,Chen Wenjun,Li Honghua.EFFECTS OF GANGLIOSIDE GM1 ON APOPTOSIS AND BCL-2 AND BAX EXPRESSIONS OF SUBSTANTIA NIGRA NEURONS IN MICE WITH PARKINSON'S DISEASE[J].Chinese Journal of Histochemistry and Cytochemistry,2007,16(4):474-477. |
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| Authors: | Wu Le Liu Zijian Chen Wenjun Li Honghua |
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| Institution: | Department of Neurology;Wuhan General Hospital of the Guangzhou Command of PLA;Wuhan 430070;1Department of Anatomy Tongji Medical College;Huazhong University of Science and Technology;Wuhan 430030;China |
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| Abstract: | Objective To investigate the effects of ganglioside GM1 on the apoptosis and the Bcl-2 and Bax expressions of substantia nigra (SN) neurons in mice with Parkinson's disease (PD). Methods Adult C57-BL male mice were randomly dividedly into 3 groups: the control group (ConG), PD model group (PDG) and ganglioside GM1 group (GM1G). By using TUNEL and immunofluorenscence staining the apoptosis and the Bcl-2 and Bax expressions of SN neurons were examined in all groups. Results Morphological examination showed th... |
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| Keywords: | Ganglioside GM1 Parkinson's disease Apoptosis Bax Bcl-2 |
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