| 大鼠脑缺血再灌注损伤后神经元和星形胶质细胞的动态变化及Cyclin D1表达的差异 |
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| 引用本文： | 朱丽霞,席青松,王晶,陈华先,王芳,张琼,王伟.大鼠脑缺血再灌注损伤后神经元和星形胶质细胞的动态变化及Cyclin D1表达的差异[J].中国组织化学与细胞化学杂志,2008,17(4):315-319. |
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| 作者姓名： | 朱丽霞 席青松 王晶 陈华先 王芳 张琼 王伟 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院神经科,武汉,430030 |
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| 摘 要： | 目的研究大鼠局灶性脑缺血再灌注损伤后神经元和星形胶质细胞表达量的动态演变及各自cyclin D1的表达差异。方法建立大鼠大脑中动脉阻塞（MCAO）再灌注模型，随机分为再灌注后1d组，3d组，7d组，14d组和假手术组，应用流式细胞术检测各组再灌注后不同时间点神经元和星形胶质细胞数量变化及各自cyclin D1的表达。结果缺血侧梗死边缘区皮质星形胶质细胞的表达增加，而神经元的表达下降，与假手术组比较有明显差异（P〈0．05）；神经元和星形胶质细胞中各自cyclin D1的表达在再灌注7d、14d后表达上调，且星形胶质细胞中的cyclinD1增加更明显，与假手术组比较有统计学差异（P〈0．05）。结论大鼠脑缺血再灌注后，缺血侧梗死边缘区皮质星形胶质细胞和神经元的cyclinD1表达均有不同程度的上调，星形胶质细胞的cyclin D1表达上调比神经元的更为显著。
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| 关 键 词： | 大脑中动脉阻塞 cyclin D1 神经元 星形胶质细胞 流式细胞术 |
THE DYNAMIC CHANGES OF NEURONS AND ASTROCYTES AND THEIR CYCLIN D1 EXPRESSION DIFFERENCE IN RATS AFTER CEREBRAL ISCHEMIA/REPERFUSION INJURY |
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| Zhu Lixia,Xi Qingsong,Wang Jing,Chen Huaxian,Wang Fang,Zhang Qiong,Wang Wei.THE DYNAMIC CHANGES OF NEURONS AND ASTROCYTES AND THEIR CYCLIN D1 EXPRESSION DIFFERENCE IN RATS AFTER CEREBRAL ISCHEMIA/REPERFUSION INJURY[J].Chinese Journal of Histochemistry and Cytochemistry,2008,17(4):315-319. |
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| Authors: | Zhu Lixia Xi Qingsong Wang Jing Chen Huaxian Wang Fang Zhang Qiong Wang Wei |
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| Institution: | Zhu Lixia,Xi Qingsong,Wang Jing,Chen Huaxian,Wang Fang,Zhang Qiong,Wang Wei(Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan,430030,China) |
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| Abstract: | Objective To study the change in the expressions of astrocytes and neurons of rats after focal cerebral ischemia and the difference in the expression of cyclin D1,respectively.Methods Ischemia was induced by temporary middle cerebral artery occlusion(MCAO).The experimental rats suffering from MCAO were sacrificed on days 1,3,7 and 14 after reperfusion.The brain was taken and the expressions of astrocytes and neurons,and cyclin D1 expression in astrocytes and neurons were respectively detected by flow cytome... |
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| Keywords: | cyelin D1 |
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