| Balb/C乳鼠小脑Purkinje细胞原代培养方法 |
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| 引用本文： | 余姗姗,马丽丽,徐善全,陈博,卜碧涛.Balb/C乳鼠小脑Purkinje细胞原代培养方法[J].中国组织化学与细胞化学杂志,2010,19(2):131-134. |
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| 作者姓名： | 余姗姗 马丽丽 徐善全 陈博 卜碧涛 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院神经内科,武汉,430030 |
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| 摘 要： | 目的建立一种简单、有效的Balb/C乳鼠小脑Purkinje细胞原代培养方法,并初步研究其细胞电生理特性。方法钝性分离生后24h内的乳鼠小脑,采用低浓度胰蛋白酶加机械吹打法获得单细胞悬液,然后用含10%胎牛血清的DMEM/F12培养,24h候更换为1%N2+1%T3+1%谷氨酰胺的DMEM/F12维持培养,3-5d天后进行细胞免疫荧光染色,7-9d天后用全细胞膜片钳单通道法记录钙电流。结果该方法培养的神经元形态典型,细胞学鉴定阳性率高(70%),并能记录到钙通道电流。结论该方法取材容易,操作简便,且培养出的原代Purkinje细胞生长状态较好,神经元的活性较高,可用于电生理研究。
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| 关 键 词： | 原代培养 Purkinje细胞 小脑 Balb/C乳鼠 |
METHOD OF PRIMARY CULTURE FOR CEREBELLAR PURKINJE CELLS OFNEONATAL Balb/C MICE |
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| Yu Shanshan,Ma Lili,Xu Shanquan,Chen Bo,Bu Bitao.METHOD OF PRIMARY CULTURE FOR CEREBELLAR PURKINJE CELLS OFNEONATAL Balb/C MICE[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(2):131-134. |
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| Authors: | Yu Shanshan Ma Lili Xu Shanquan Chen Bo Bu Bitao |
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| Institution: | Yu Shanshan,Ma Lili,Xu Shanquan,Chen Bo,Bu Bitao(Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China) |
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| Abstract: | Objective To establish an easier and better primary cell culture method suitable for cerebellar cortex Purkinje cells of neonatal Balb/C mice,and preliminaril research the cytoelectrophysiological characteristics of the cultured cells.Methods Cerebella of neonatal mice born within 24 hours were dissected bluntly to obtain suspended solution of single cells by low-concentration trypsin digestion and mechanical dissociation,then the cells were cultured in DMEM/F12 media containing 10% fetal bovine serum.The m... |
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| Keywords: | Primary Culture Purkinje Cell Cerebellar Balb/C Newborn Mouse |
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