EZH2和CBX7在皮肤血管瘤中的异常表达及意义

目的探讨EZH2和CBX7在血管瘤中的的异常表达及临床意义。方法收集武汉大学人民医院病理科2005年9月一2009年12月皮肤毛细血管瘤存档蜡块50例，其中男性25例，女性25例。采用免疫组织化学S-P法检测50例皮肤血管瘤增生期、退化期及正常皮肤组织中EZH2和CBX7的表达水平，采用图像分析系统对EZH2和CBX7的表达进行定量分析，并用SPSS13．0软件对各组免疫组织化学反应阳性颗粒的平均光密度、阳性面积率做单因素方差分析和SNK（q）检验。结果增生期血管瘤血管内皮细胞中可见密集分布的棕黄色颗粒，EZH2和CBX7呈高表达，退化组及正常皮肤组血管内皮细胞中可见少量的棕黄色颗粒，EZH2和CBX7表达弱。增生期组EZH2和CBX7的表达明显高于退化期组和正常皮肤组（P〈0．05），而后两组比较差异无统计学意义（P〉0．05）。结论EZH2和CBX7在血管瘤增生期均上调表达，表明EZH2和CBX7均与血管瘤内皮细胞的增殖密切相关。

Abnormal expression and clinical significance of EZH2 and CBX7 in Human Dermal Hemangiomas

Objective To study the effect of EZH2 and CBX7 in the development of human dermal hemangioma. Methods Specimens from 50 cases （25 males, and 25 females） of hemangioma from the Department of Pathology in Renmin Hospital of Wuhan University from 2005 to 2009 were collected. We examined the expression of EZH2 and CBX7 in proliferative, involuting hemangiomas and normal skin tissues by using immunohistochemical technique. Average optical density and positive area rates of expression of EZH2 and CBX7 were measured by image analysis （HPIAS-1000）. Results EZH2 and CBX7 expressions were significanthy higher in the proliferative phase of hemangiomas, than in involuting hemangi- oma and normal skin tissues（P〈 0.05）. No significant differences were found between the expressions of CBX7 and EZH2 in involuting hemangioma and those in normal skin tissues （P〉0. 05）. Conclusion EZH2 and CBX7 are up-regulated, in proliferating hemangioma which indicates that both EZH2 and CBX7 are closely related to the vascular tumor occurrence and development.