| 铈代替铅电镜酶组织化学显示培养脊神经节神经元TMP活性 |
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| 引用本文： | 刘冬娟,徐彦平,石玉秀.铈代替铅电镜酶组织化学显示培养脊神经节神经元TMP活性[J].中国组织化学与细胞化学杂志,2011,20(1):53-55. |
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| 作者姓名： | 刘冬娟 徐彦平 石玉秀 |
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| 作者单位： | 中国医科大学基础医学院电子显微学研究室,沈阳,110001 |
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| 摘 要： | 目的探讨铈法显示脊神经节神经元初代培养细胞的TMP(三偏磷酸酶)活性。方法应用电镜酶组化技术,以铈作为捕捉剂代替常规以铅作为捕捉剂来检测观察脊神经节初代培养的神经元内溶酶体的TMP活性。结果脊神经节神经元初代培养细胞内存在TMP阳性的溶酶体,其TMP阳性反应颗粒较常规以铅作为捕捉剂来显示溶酶体不仅孵育液充分溶解呈清亮透明,电镜下反应产物颗粒也微细均匀,而且避免了反应产物的扩散及非特异性沉淀。结论以铈作为捕捉剂来检测溶酶体的TMP活性用于电镜下观察,其方法稳定、易行,是一种能很好显示溶酶体标记酶的电镜酶组化技术方法。
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| 关 键 词： | 铈 脊神经节 神经元 三偏磷酸酶 电镜组织化学 |
Etectron microscopic enzyme histochemistry using cerium as a substitule for lead to demonstrate TMP activity in the spinal ganglionic neurons |
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| Liu Dongjuan,Xu Yanping,Shi Yuxiu.Etectron microscopic enzyme histochemistry using cerium as a substitule for lead to demonstrate TMP activity in the spinal ganglionic neurons[J].Chinese Journal of Histochemistry and Cytochemistry,2011,20(1):53-55. |
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| Authors: | Liu Dongjuan Xu Yanping Shi Yuxiu |
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| Institution: | Liu Dongjuan,Xu Yanping,Shi Yuxiu(Department of Electron Microscopy,China Medical University,Shenyang 110001,China) |
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| Abstract: | Objective To investigate the cerium method showing the TMP(thiamine monophosphatase) positive neurons in the primative culture of spinal ganglion cells.Methods Applying electron microscopic histochemistry,we used cerium instead of conventional lead as the capture agent to detect the TMP activity in the lysosome of cultured ganglion neurons.Results In the spinal ganglion cells,lysosomes were positively stained with TMP.The novel cerium agent was completely soluable in the culture medium.The particles under t... |
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| Keywords: | Cerium Cerebrospinal ganglia Neuron Thiamine monophosphatase Electron microscopic histochemistry |
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