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| PDCD4基因在过氧化氢诱导喉癌细胞凋亡中的作用 | |
| 引用本文： | 李林,陈晓锐,宋莎莎,逯素梅,张国栋,李建峰.PDCD4基因在过氧化氢诱导喉癌细胞凋亡中的作用[J].中国组织化学与细胞化学杂志,2010,19(1):72-76. |
| 作者姓名： | 李林 陈晓锐 宋莎莎 逯素梅 张国栋 李建峰 |
| 作者单位： | 1. 山东大学附属省立医院中心实验室,济南,250021;山东省临床医学研究院眼耳鼻喉科学研究所,济南,250022 2. 滨州医学院医学人文学院,烟台,264003 3. 山东省临床医学研究院眼耳鼻喉科学研究所,济南,250022 |
| 基金项目： | 山东省科技厅资助(2006GG2202) |
| 摘 要： | 目的探讨过氧化氢诱导喉癌细胞Hep-2凋亡过程中PDCD4基因表达的变化。方法以体外培养的喉癌细胞Hep-2为实验材料,不同浓度的过氧化氢作用于Hep-2细胞,噻唑蓝(MTT)比色法测定细胞生存率,采用吖啶橙染色、Ho33342/PI荧光双染进行形态学观察,RT-PCR及Western blot检测PDCD4 mRNA水平及蛋白表达的变化,评价在过氧化氢诱导喉癌细胞Hep-2凋亡过程中PDCD4基因的作用。结果过氧化氢(200μmol/L)作用Hep-2细胞24h,能够显著抑制细胞增殖,并诱导细胞凋亡,同时引起pdcd4 mRNA水平显著上调,PDCD4蛋白表达显著增加。结论本研究首次报道PDCD4基因可能在氧化胁迫诱导喉癌细胞凋亡中起关键作用。 |
| 关 键 词： | PDCD4 Hep-2 细胞凋亡 氧化应激 喉癌 |
ROLE OF PDCD4 GENE IN HYDROGEN PEROXIDE-INDUCED APOPTOSIS IN LARYNGEAL CARCINOMA HEP-2 CELLS |
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| Li Lin,Chen Xiaorui,Song Shasha,Lu Sumei,Zhang Guodong,Li Jianfeng.ROLE OF PDCD4 GENE IN HYDROGEN PEROXIDE-INDUCED APOPTOSIS IN LARYNGEAL CARCINOMA HEP-2 CELLS[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(1):72-76. | |
| Authors: | Li Lin Chen Xiaorui Song Shasha Lu Sumei Zhang Guodong Li Jianfeng |
| Institution: | 1Central Lab;Provincial Hospital Affiliated to Shandong University;Jinan;250021;2 Medical College of Binzhou;Yantai;264003;P.R.China;3 Institute of Eye & Otolaryngology;Shandong Clinic Research Institute;250022 China |
| Abstract: | Objective To investigate the change of PDCD4 gene expression in laryngeal carcinoma Hep-2 cells in response to the oxidative stress by hydrogen peroxide (H2O2).Methods Hep-2 cells were cultured in vitro and subjected to different concentrations of H2O2. Cell viability was measured by MTT assay. The morphological changes were assessed by acridine orange staining and Ho33342/PI double staining to determine whether the cells experienced apoptosis under the oxidative stress. In particular, the changes in the ex... |
| Keywords: | PDCD4 gene Hep-2 cell Apoptosis Oxidative stress Larynx carcinoma |
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