| 融合热释放对低温冷冻人骨髓细胞的核酸与酶类活性的影响 |
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| 引用本文： | 高玉民,梁丽华,谢艳,马久才.融合热释放对低温冷冻人骨髓细胞的核酸与酶类活性的影响[J].中国组织化学与细胞化学杂志,1993(4). |
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| 作者姓名： | 高玉民 梁丽华 谢艳 马久才 |
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| 作者单位： | 空军总医院血液病学实验室，空军总医院血液病学实验室，空军总医院血液病学实验室，空军总医院血液病学实验室 北京 100036，北京 100036，北京 100036，北京 100036 |
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| 摘 要： | 本文用定性定量组织细胞化学方法,对冻前及不同降温条件冻存的人骨髓细胞的 DNA、碱性磷酸(ALP)、过氧化物酶(POX)活性进行测定。在程序降温过程中,没有消除融合热的骨髓细胞的 DNA、ALP、POX 活性均显著下降。消除融合热后,冻存骨髓细胞 DNA 活性没有改变,ALP、POX 活性略有下降。因而,融合热的释放是骨髓细胞生物活性下降的重要因素。
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| 关 键 词： | 融合热 低温冷冻 骨髓细胞核 酸与酶活性 |
INFLUENCE OF FUSIN HEAT RELEASE ON ACTIVATION OF NUCLEIC ACID AND ENZYMES IN CRYOPRESERVATED HUMAN MARROW CELLS |
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| Gao Yumin Liang Lihua xie Yan Ma Jiudai.INFLUENCE OF FUSIN HEAT RELEASE ON ACTIVATION OF NUCLEIC ACID AND ENZYMES IN CRYOPRESERVATED HUMAN MARROW CELLS[J].Chinese Journal of Histochemistry and Cytochemistry,1993(4). |
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| Authors: | Gao Yumin Liang Lihua xie Yan Ma Jiudai |
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| Abstract: | Activation of deoxyribonucleic acid(DNA),alkaline phosphatase(ALP)and peroxidase (POX)of human marrow cells were measured under prefreezing and different freezing condi- tions by using quantitative and qualitative histochemistry method.In the course of ordinary freezing,the activation of DNA.ALP and POX in human marrow cells were remarkably re- duced when fusion heat of freezing bone marrow cells wasnlt removed.After removing of fu- sion heat of freezing bone marrow cells the activation of DNA in cryopreservated human mar- row cells remained unchanged but that of ALP and POX in then reduced a little reduce. These results showed that the fusion heat release of cryopreservated marrow cells was a ma- jor reason for the reduction of biological activation in marrow cells. |
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| Keywords: | Fusion heat Cryopreservation Marrow cells Nucleic acid and enymes |
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