地塞米松对哮喘豚鼠肺内VIP分布的影响

为探讨哮喘豚鼠肺内血管活性肠肽（ＶＩＰ）的分布及地塞米松对其分布的影响，将豚鼠随机分成哮喘组、地塞米松组和对照组三组，采用兔抗ＶＩＰ多克隆血清，免疫组织化学ＡＢＣ法和葡萄糖氧化酶－ＤＡＢ－镍染色技术，发现对照豚鼠肺内各级气道壁均可见ＶＩＰ免疫反应（ＶＩＰ－ＩＲ）阳性纤维分布。在平滑肌层中，纤维成束走行，且有较多分枝；而在基底膜和气道上皮内，纤维成单根行走。随气道口径的变小，纤维分布密度也逐渐变小。哮喘豚鼠肺内ＶＩＰ－ＩＲ消失。地塞米松处理后使ＶＩＰ－ＩＲ部分恢复。上述结果提示，豚鼠经反复抗原攻击后，肺内ＶＩＰ－ＩＲ阳性纤维消失，这在实验性哮喘的发生过程中可能起一定作用。

INFLUENCE OF DEXAMETHASON ON THE DISTRIBUTION OF VIP IN THE LUNGS OF GUINEA PIGS SUFFERING FROM ASTHMA

ntiserum against VIP, immunohistochemistry and gluco oxidase DAB neckle technique for staining were used in this study to investigate the distribution of VIP in the lungs of guinea pigs suffering from asthma and the influence of dexamethason on the distribution. The guinea pigs were divided into 3 group(i.e., asthma group, control group and dexamethason treated group). The results showed that the VIP IR positive fibres were seen in the airways down to the bronchioles in lungs from control group. The fibres were commonly seen as bundle fibres with branches in smooth muscle layer and as single fibres in the basement membrance and epithelium. No VIP IR was seen in the lungs from asthma group. After asthma guinea pigs were treated with dexamethason, VIP IR reappeared in lungs. After they were repeatly challenged with allergen, the VIP IR positive nerve fibres disappeared. This may be correlated with the pathogenisis of asthma.