| 人脊髓前角运动神经元190KD蛋白的初步研究 |
| |
| 引用本文： | 张沛云,顾晓松,张爱国,曹铮,严志强,谭湘陵.人脊髓前角运动神经元190KD蛋白的初步研究[J].中国组织化学与细胞化学杂志,1998(4). |
| |
| 作者姓名： | 张沛云 顾晓松 张爱国 曹铮 严志强 谭湘陵 |
| |
| 作者单位： | 南通医学院神经科学研究所 |
| |
| 摘 要： | 本研究采用ＳＤＳ凝胶电泳方法从人脊神经前根中分离出人脊髓前角运动神经元特有的蛋白—１９０ＫＤ。将该蛋白作为抗原，免疫ＢＡＬＢ／ｃ小鼠，经杂交瘤技术，获得了抗１９０ＫＤ蛋白的单克隆抗体。免疫细胞化学检测表明，１９０ＫＤ单抗与脊髓灰质前角神经元、前根及肌支发生阳性反应。实验结果提示，１９０ＫＤ蛋白分布在脊髓运动神经元胞体及脊神经的前根和肌支纤维中。
|
| 关 键 词： | 运动神经元 190KD蛋白 脊髓前角 单克隆抗体 人 |
A PRELIMIARY STUDY OF A 190KD PROTEIN OF MOTONEURONS IN ANTERIOR HORN OF HUMAN SPINAL CORD |
| |
| Zhang Peiyun,Gu Xiaosong,Zhang Aiguo,Gao Zheng,Yan Zhiqiang,Tan Xiangling.A PRELIMIARY STUDY OF A 190KD PROTEIN OF MOTONEURONS IN ANTERIOR HORN OF HUMAN SPINAL CORD[J].Chinese Journal of Histochemistry and Cytochemistry,1998(4). |
| |
| Authors: | Zhang Peiyun Gu Xiaosong Zhang Aiguo Gao Zheng Yan Zhiqiang Tan Xiangling |
| |
| Abstract: | A 190KD protein of motoneurons was isolated from the anterior roots of human spinal cord by SDS PAGE. The monoclonal antibody was prepared through immunizing the BALB/c mouse with 190KD protein. The results of the immunohistochemistry showed that positive products distributed in motoneurons in anterior horn of spinal cord, the fibers of anterior roots, and muscular branches of peripheral nerve. This study suggests that the 190KD protein may be a specific protein for motoneurons, which exists only in the motoneurons, anterior roots and muscular branches. |
| |
| Keywords: | Motoneuron 190KD protein Anterio horn Spinal cord Monoclonal antibody Human |
| 本文献已被 CNKI 等数据库收录！ |
