大鼠全脑缺血后纹状体边缘区内降钙素基因相关肽和谷氨酸脱羧酶表达的变化

为研究全脑缺血再灌流后纹状体边缘区内降钙素基因相关肽（CGRP）和谷氨酸脱羧酶（GAD）等神经递质的表达变化。在两侧颈总动脉和椎动脉结扎造成的大鼠全脑缺血模型上，用免疫组织化学方法观察纹状体边缘区内CGRP和GAD等免疫阳性反应的分布，结果显示正常大鼠脑纹状体内有CGRP和GAD阳性神经纤维的分布，全脑缺血再灌流1天后，边缘区中的CGRP免疫阳性反应逐渐增强，在第5天时达到最高峰，全脑缺血再灌流后1天，可见边缘区部位的GAD免疫阳性反应增强，但以后边缘区内的GAD免疫反应阳性物质逐渐减少，缺血再灌流5天后，边缘区中GAD免疫阳性反应的表达基本消失。研究结果表明全脑缺血后边缘区中的CGRP反应逐渐增强，这种变化可能和脑缺血后动物的学习记忆能力下降有关。

EXPRESSION OF CALCITONIN GENE-RELATED PEPTIDE (CGRP) AND GLUTAM IC ACID DECARBOXYLASE (GAD) IN RAT STRIATUM FOLLOWING GLOBAL ISCHEMIA

To investigate the expresson changes of calcitonin gene related peptide (CGRP) and glutamic acid decarboxylase (GAD) in the rat striatum following global ischemia, immunohistochemical methods were used to examine the distribtution of CGRP and GAD in the rat striatum following global ischemia on the 4 vessel ligation models. The results showed that there are CGRP and GAD positive fibers in the normal rat striatum. The immunoreactivity of CGRP in the marginal division was increased aftef blobal ischemia and reperfusion, and reached the maximum on the 5th day after global ischemia and reperfusion. The immunoreactivity of GAD in the marginal division was increased on the first day, then decreased gradually and disappeared on the 5th day after global ischemia and reperfusion. It may be concluded that the immunoreactivity of CGRP in the marginal division is increased after global ischemia and reperfusion and this change may be related to the attenuation of learning capacity and memory following global ischemia.