| 热休克蛋白HSP70在LPS诱导急性肺损伤中的表达 |
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| 引用本文： | 于东云,金抗,石玉秀.热休克蛋白HSP70在LPS诱导急性肺损伤中的表达[J].中国组织化学与细胞化学杂志,2006,15(3):280-283. |
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| 作者姓名： | 于东云 金抗 石玉秀 |
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| 作者单位： | 1. 中国医科大学第一临床学院急诊科,沈阳,110001
2. 中国医科大学组胚教研室,沈阳,110001 |
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| 摘 要： | 目的检测内毒素诱导急性肺损伤中热休克蛋白70的表达状况,探讨HSP70在急性肺损伤中的作用机制。方法在LPS致Wistar大鼠急性肺损伤动物模型上,采用免疫组织化学(SABC法)和蛋白印迹实验研究各组动物肺组织中HSP70的表达情况。结果LPS处理后1h,大鼠的支气管粘膜上皮及肺泡上皮细胞HSP70的表达与正常对照组相比明显增多,2h的表达达到高峰,6h后与对照组水平一致。Western blot结果显示,LPS处理2h、4h时,HSP70的表达较对照组明显增强。结论LPS诱导的急性肺损伤中可引起支气管、细支气管和肺泡上皮细胞HSP70应激性表达,提示HSP70对肺损伤起保护作用。
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| 关 键 词： | 脂多糖 急性肺损伤 热休克蛋白70 大鼠 |
| 收稿时间： | 10 9 2005 12:00AM |
| 修稿时间： | 11 30 2005 12:00AM |
EXPRESSION OF HSP70 IN ACUTE LUNG INJURY INDUCED BY LPS |
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| Yu Dongyun,Jin Kang,Shi Yuxiu.EXPRESSION OF HSP70 IN ACUTE LUNG INJURY INDUCED BY LPS[J].Chinese Journal of Histochemistry and Cytochemistry,2006,15(3):280-283. |
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| Authors: | Yu Dongyun Jin Kang Shi Yuxiu |
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| Institution: | Department of Emergency Medicine, The First Affiliated Hospital, China Medical University ;1 Department of Histology and Embryology, China Medical University, Shengyang 110001, China |
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| Abstract: | Objective To examine the expression of HSP70 in acute lung injury(ALI) induced by LPS,in order to explore the mechanism of HSP70 in ALI.Methods We observed in vivo the HSP70 expression by immunohistochemistry and Western Blot.Results Immunohistochemical staining showed that the level of HSP70 increased at 1 hour after treatment with LPS,reached the maximum level at 2 hours,and then decreased to the level of the control group at 6 hour.The expressions of HSP70 at each time point were shown by Westrn Blotting.Conclusion The results suggest that HSP70 may play a role in the protection of ALI induced by LPS. |
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| Keywords: | LPS Acute lung injury HSP70 Rat |
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