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| 褪黑素对胃癌小鼠Survivin表达的影响 | |
| 引用本文： | 顾良红,刘卉,魏建恩,于浩,卢旭东,陈国红,周瑞祥.褪黑素对胃癌小鼠Survivin表达的影响[J].中国组织化学与细胞化学杂志,2010,19(6):583-586. |
| 作者姓名： | 顾良红 刘卉 魏建恩 于浩 卢旭东 陈国红 周瑞祥 |
| 作者单位： | 1. 三明职业技术学院护理系医学基础教研室,福建,365000;福建医科大学人体解剖学与组织胚胎学系,福州,350004 2. 福建医科大学人体解剖学与组织胚胎学系,福州,350004 3. 三明职业技术学院护理系医学基础教研室,福建,365000 |
| 基金项目： | 国家自然基金资助项目(3097154); 福建省科技厅重点项目(2009Y0023) |
| 摘 要： | 目的探讨褪黑素对胃癌小鼠Survivin表达的影响。方法选用6-8周龄SPF级615近交系小鼠,接种胃癌MFC细胞,建立荷瘤小鼠模型并给予不同剂量的褪黑素处理,用Real-time PCR法和Western blot法检测肿瘤组织中Survivin mRNA和蛋白的表达。结果 Real-time PCR和Western-blot检测显示褪黑素各干预组小鼠胃癌组织中Survivin基因的表达水平下降且随褪黑素剂量增大而降低。结论褪黑素具有下调survivin的作用。褪黑素降低survivin的表达,可能是褪黑素抑制肿瘤的作用机制之一。 |
| 关 键 词： | 褪黑素 胃癌 Survivin |
EFFECTS OF MELATONIN ON SURVIVIN EXPRESSION IN MICE WITH GASTRIC CANCER |
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| Gu Lianghong,Liu Hui,Wei Jianen,Yu Hao,Lu Xudong,Chen Guohong,Zhou Ruixiang.EFFECTS OF MELATONIN ON SURVIVIN EXPRESSION IN MICE WITH GASTRIC CANCER[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(6):583-586. | |
| Authors: | Gu Lianghong Liu Hui Wei Jianen Yu Hao Lu Xudong Chen Guohong Zhou Ruixiang |
| Institution: | Gu Lianghong1,2,Liu Hui2,Wei Jianen2,Yu Hao1,Lu Xudong1,Chen Guohong1,Zhou Ruixiang2(1Department of Nursing,Sanming Vocational Technical College,Fujian 365000,2Department of Anatomy,Histology and Embryology,Fujian Medical University,Fuzhou 350004,China) |
| Abstract: | Objective To study the effect of melatonin on Survivin expression in mice with gastric cancer.Methods 615 SPF-class inbred mice of 6-8 weeks were selected.Animal models were established by transplanting MFC gastric cancer cells and treated with melatonin.Survivin mRNA and protein expression in the tumor tissues was detected by Real-time PCR and Western blot.Results Western-blot and Real-time PCR analysis showed that the groups dosed with melatonin gave lower expression levels of Survivin gene,which decrease... |
| Keywords: | Melatonin Gastric Cancer Survivin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |