人原发性肝细胞肝癌中丙型肝炎病毒C_（33c）抗原及HBxAg的分布及意义

作者应用抗ＨＣＶＮＳ３区Ｃ３３ｃ抗原２Ｂ６株单克隆抗体和抗ＨＢｘＡｇ多克隆抗体，采用ＡＢＣ法对１０２例人原发性肝细胞肝癌（ＰＨＣ）组织进行了ＨＣＶ及ＨＢＶ抗原定位研究。ＨＣＶＣ３。抗原及ＨＢｘＡｇ在ＰＨＣ中的阳性检出率分别是８１．４％及７４．５％，Ｃ３３ｃ抗原或ＨＢｘＡｇ阳性占所检病例９４．１％，相同病例二者同时阳性为６１．８％。１０２例ＰＨＣ中５０例有癌旁肝组织，其Ｃ３３ｃ抗原和ＨＢｘＡｇ的阳性检出率分别是６２％和９２％。ＨＣＶＣ３３ｃ抗原定位于肝癌细胞的胞浆内，胞核未见阳性信号。Ｃ３３ｃ抗原阳性细胞在ＰＨＣ中呈散在、局灶分布为主，在癌旁肝组织呈弥漫分布为主。本文结果提示ＨＣＶ感染在ＰＨＣ的发生中可能起重要作用。

DISTRIBUTION AND SIGNIFICANCE OF HCV C33c ANTIGEN AND HBxAg IN PRIMARY HEPATOCELLULAR CARCINOMA

In order to explore the relationship between HCV infection and primary hepatocellular carcinoma(PHC),HCV NS3 region C33c antigen and HBxAg were detected with monoclonal antibody to C33c antigen and polyclonal antibody to HBxAg by immunohistochemistry method in paraffin-embedded sections of PHC tissues.in 102 cases The positive rate of HCV C33cantigen and HBxAg was 81.4%(33/102) and 74.5%(76/102) respectively.The overall detection rate of HCV C33c and HBxAG was 94.1% (96/102).The double positive rate of HCVC33c and HBxAg was 61.3%(63/102).Among 102 PHC cases,50 cases were with nontumorous liver tissues,in which positive rate of HCV C33c antigen and HBxAg in it was 64%(32/50) and 92%(46/50) respectively.HCV C33c antigen was clearly stained in the cytoplasm of cancerous cell and hepatocyte and not in the nucleus or cell membrane.The positive substance was distributed locally or diffusely in the cytoplasm of cancerous cell.Three main types of distribution pattern of HCV C33c antigen positive cell were observed.They were patchy.cluster and diffuse.The main pattern in PHC was patchy and cluster,which that in nontumorous liver tissues was diffuse.Our results suggest that HCV infection is very common in PHC patients in Xi'an area.and it may play an important role in the development of PHC except HBV infection.