| 黄芩甙对肝癌细胞BEL-7402形态学的影响 |
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| 引用本文： | 郭昱,郭霞,姚金峰,崔东来,姚树坤.黄芩甙对肝癌细胞BEL-7402形态学的影响[J].中国组织化学与细胞化学杂志,2008,17(2):174-179. |
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| 作者姓名： | 郭昱 郭霞 姚金峰 崔东来 姚树坤 |
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| 作者单位： | 1. 河北医科大学第二医院消化内科,石家庄,050000
2. 河北医科大学第四医院,石家庄,050011 |
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| 摘 要： | 目的观察黄芩甙对肝癌细胞BEL-7402凋亡的影响，同时观察对肝癌细胞形态及超微结构、线粒体超微结构、线粒体膜电位和细胞内Ca^2＋的影响，探讨线粒体损伤在黄芩甙诱导肝癌细胞凋亡中的作用及可能的机制。方法应用细胞培养技术培养肝癌细胞BEL-7402，光镜、倒置显微镜、扫描电镜、透射电镜观察细胞形态及超微结构的变化尤其是线粒体的变化，应用流式细胞仪检测细胞凋亡百分率及线粒体膜电位、细胞内Ca^2＋的改变，免疫组化法检测细胞Bcl-2、Pax蛋白表达。结果黄芩甙诱导肝癌细胞BEL-7402凋亡呈剂量依赖关系，细胞形态、超微结构及线粒体超微结构出现明显改变，降低肝癌细胞线粒体膜电位，使细胞内Ca^2＋增加，细胞Pax表达增加，广泛分布于胞核和胞质中，Bcl-2表达减少。结论黄芩甙诱导肝癌细胞BEL-7402凋亡，线粒体损伤在黄芩甙诱导肝癌细胞凋亡中起重要作用，其机制可能为抑制肝癌细胞Bcl-2蛋白表达，促进Pax蛋白表达及细胞内Ca^2＋增加，激发线粒体膜通透性转运孔开放，线粒体跨膜电位降低，使肝癌细胞凋亡。
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| 关 键 词： | 黄芩甙 肝细胞癌 超微结构 凋亡 线粒体膜电位 |
| 修稿时间： | 2007年8月8日 |
EFFECT OF BAICALIN ON ULTRASTRUCTURE OF HCC CELL LINE BEL-7402 |
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| Guo Yu,Guo Xia,Yao Jinfeng,Cui Donglai,Yao Shukun.EFFECT OF BAICALIN ON ULTRASTRUCTURE OF HCC CELL LINE BEL-7402[J].Chinese Journal of Histochemistry and Cytochemistry,2008,17(2):174-179. |
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| Authors: | Guo Yu Guo Xia Yao Jinfeng Cui Donglai Yao Shukun |
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| Institution: | Guo Yu1,Guo Xia2,Yao Jinfeng1,Cui Donglai1,Yao Shukun2(1Department of Gastroenterology,The Second Hospital of Hebei Medical University,Shijiazhuang 050000,2 Department of Internal Medicine,The Fourth Hospital ofHebei Medical University,Shijiazhuang 050011,China) |
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| Abstract: | Objective To investigate the changes in morphology,ultrastructure,mitochondria membrane potential and intracellular Ca2+ of hepatocellular carcinoma(HCC)cell line BEL-7402 after treatment with baicalin,in order to explore the anti-tumour mechanism of baicalin.Methods HCC cell line BEL-7402 was cultured.The changes of morphology and ultrastructure were observed by light,inverted and electron microscopy.Mitochondria membrane potential,intracellular Ca2+ as well as apoptosis rate were determined by using flow ... |
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| Keywords: | Hepatocellular carcinoma Baicalin Ultrastructure Apoptosis Mitochondria membrane potential |
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