| 慢性缺氧大鼠肺动脉一氧化氮合酶与蛋白激酶C表达变化关系的免疫组织化学研究 |
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| 引用本文： | 钟声,徐永健,张珍祥.慢性缺氧大鼠肺动脉一氧化氮合酶与蛋白激酶C表达变化关系的免疫组织化学研究[J].中国组织化学与细胞化学杂志,2000,9(3):248-250. |
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| 作者姓名： | 钟声 徐永健 张珍祥 |
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| 作者单位： | 湖北省黄石市中心医院呼吸内科!435000(钟声)，同济医科大学附属同济医院呼吸内科!武汉430030(徐永，张珍祥) |
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| 基金项目： | 国家自然科学基金!(No. 39770 34 1)，国家教委留学回国人员科研启动金!(No. [1997]437)项目资助课题 |
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| 摘 要： | 观察慢性缺氧对大鼠肺动脉诱导型一氧化氮合酶 (i NOS)和蛋白激酶 C (PKC)表达的影响 ,并探讨 i NOS及 PKC的相互关系在肺动脉高压形成中的作用。建立常压缺氧肺动脉高压模型。应用免疫组化染色和计算机定量图像分析技术观察各组 i NOS和 PKC在蛋白质水平表达的变化 ,并分析二者之间的关系。结果表明随着缺氧时间的延长 ,PKC的表达逐渐上升而 i NOS的表达逐渐下降 ,直线相关分析结果呈显著性负相关 (n=2 4,r=- 0 .94,P<0 .0 1) ,提示 i NOS与 PKC相互拮抗作用与缺氧性肺动脉高压的形成及发展有关
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| 关 键 词： | 表达变化 免疫组织化学 蛋白激酶C 一氧化氮合酶 缺氧性肺动脉高压 大鼠 |
THE IMMUNOHISTOCHEMICAL STUDY OF THE CHANGE OF THE RELATION OF NITRICE OXIDE SYNTHASE AND PROTEIN KINASE C EXPRESSION ON RAT PULMONARY ARTERISE FROM CHRONIC HYPOXIA |
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| Zhong Sheng,Xu Yongjian,Zhang Zhengqiang.THE IMMUNOHISTOCHEMICAL STUDY OF THE CHANGE OF THE RELATION OF NITRICE OXIDE SYNTHASE AND PROTEIN KINASE C EXPRESSION ON RAT PULMONARY ARTERISE FROM CHRONIC HYPOXIA[J].Chinese Journal of Histochemistry and Cytochemistry,2000,9(3):248-250. |
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| Authors: | Zhong Sheng Xu Yongjian Zhang Zhengqiang |
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| Abstract: | In order to observe the effectofchronic hypoxia on the expression of nitric oxide synthase( i NOS) and protein kinase C ( PKC) of pulmonary arteries and the relationship between i NOS and PKC on chronic hypoxia pulmonary hypertension ( HPH ) ,the immunohistochemical and computer- imaging analytic technique were used to observe i NOS and PKC expression on the level of protein in each rat group. The study showed positive expression of PKC gradually increased while i NOS decreased. Linear rsult is significantly negative correlated( n=2 4 ,r=- 0 .94,P<0 .0 1 ) .The study indicated thatantagonistic effect of i NOSand PKC have a bearing on the formation and development of HPH. |
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| Keywords: | Protein kinase C Induced nitric oxide synthase Hypoxic pulmonary hypertension Rat |
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