| 利用微波固定组织的收缩率 |
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| 引用本文： | 楚德昌,邓振旭,李春华,王守广,葛框框,李杰.利用微波固定组织的收缩率[J].中国组织化学与细胞化学杂志,2008,17(1):106-110. |
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| 作者姓名： | 楚德昌 邓振旭 李春华 王守广 葛框框 李杰 |
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| 作者单位： | 菏泽学院生命科学系,山东菏泽,274015 |
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| 摘 要： | 目的探讨利用微波辐射固定、微波辐射与固定液结合固定组织的收缩程度。方法将组织分成40组，用一定强度的微波辐射或一定强度的微波辐射与不同固定液结合方法固定动物组织，并制做石蜡组织切片和HE染色，在此过程中测定各步骤前后的组织块体积，计算出收缩率。结果与结论一定强度的微波辐射和一些微波辐射与固定液结合方法固定的组织收缩率低，这些组织的切片具有细胞质均匀，染色鲜艳，细胞核大而清晰的良好效果。
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| 关 键 词： | 微波 组织固定 组织收缩率 |
| 修稿时间： | 2007年5月22日 |
SHRINKAGE OF TISSUE FIXED WITH MICROWAVE |
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| Chu Dechang,Deng Zhenxu,Li Chunhua,Wang Shouguang,Ge Kuangkuang,Li Jie.SHRINKAGE OF TISSUE FIXED WITH MICROWAVE[J].Chinese Journal of Histochemistry and Cytochemistry,2008,17(1):106-110. |
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| Authors: | Chu Dechang Deng Zhenxu Li Chunhua Wang Shouguang Ge Kuangkuang Li Jie |
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| Institution: | Chu Dechang,Deng Zhenxu,Li Chunhua,Wang Shouguang,Ge Kuangkuang,Li Jie(Department of Life Sciences,Heze coclege,Heze 274015,China) |
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| Abstract: | Objective To explores the shrinkage of tissue fixed by microwave radiation alone or microwave radiation combined with some kind of fixatives.Methods The specimens were divided 40 groups,and their volume were determined after various steps of the process in which paraffin sections were made through different intensities of microwave radiation alone or microwave radiation combined with different fixatives.The rate of change of tissue volume was calcuted,and the sections were stained.Results and conclusion The... |
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| Keywords: | Microwave Tissue fixation Tissue shrinkage |
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