应用单克隆抗体CL－2、CL－4对大肠癌、癌旁病变的免疫组化研究

应用抗人结肠癌单克隆抗体ＣＬ－２，ＣＬ－４，对２０５例大肠癌及癌旁病变进行了免疫组化研究。ＣＬ－２相应抗原在移行粘膜，轻、中、重度非腺瘤异型增生，大肠癌的阳性率分别为３７．６％、６３．２％、８６．７％、９０．９％及８６．８％，阳性率呈递增趋势；ＣＬ－４相应抗原的阳性率依次是３９．１％、５７．９％、７３．３％、８１．８％及７７．６％；４０例正常大肠粘膜均阴性。结果表明，ＣＬ－２、ＣＬ－４都是对大肠癌阳性率较高的标记物，但用来鉴别癌与异型增生意义不大；一部分大肠癌可能来源于非腺瘤途径；移行粘膜不同程度的肿瘤相关抗原的表达，反映了其潜在的恶性性质，但其程度低于异型增生，文中，对其临床意义进行了讨论。

IMMUNOHISTOCHEMICAL STUDY OF COLORECTAL CARCINOMAS AND ITS SURROUNDING LESIONS BY USING MONOCLONAL ANTIBODIES CL-2,CL-4

Expression of CL-2, CL-4 monoclonal antibodies in colorectal carcinomas and its surrounding lesions was investigated by immunohistrochemistry. The positivity rates of CL-2 in transitional mucosa, mild, moderate, severe dysplasia and colorectal carcinoma were 37. 6% (76/202), 63. 2% (12/19), 86. 7% (12/15), 90. 9% (20/22) and 86. 8% (170/205) respectively. The positivity rate seemed to have an increasing tendency following the progress of mucosal lesions. The positivity rates of CL-4 in mucosal lesions of the same order were 39. 1% (79/202), 57. 9% (11/19), 73. 3% (11/15), 81. 8% (19/22) and 77. 6% (159/205) respectively, whereas the normal colorectal mucosae of 30 cases were all negative. The results indicate that CL-2, CL-4 monoclonal antibodies are markers with high positive rate for colorectal carcinomas. However, they cannot be used to differentiate dysplasia from carcinoma as the former has high positive rate too. Part of colorectal carcinomas may be derived from non-adenomas. The expressed different degree of tumor-associated antigens in transitional mucosa adjacent to the cancerous tissue reflects its potential malignant nature, but the potentiality is lower than that of mild dysplasia. its clinical importance is that the transitional mucosa may transform into malignancy through different stage of epithelial dysplasia.