非何杰金氏淋巴瘤在石蜡切片的免疫表型研究

用单克隆抗体ＬＣＡ，ＵＣＨＬ１，ＭＴ１，ＭＢ１，ＭＢ２，ＬＮ１和ＬＮ２对６５例非何杰金氏淋巴瘤常规福尔马林固定石蜡包埋组织切片作免疫表型研究。证实Ｂ细胞性淋巴瘤５０例（７７％）。Ｔ细胞性淋巴瘤１１例（１７％）（４例染色不良，未能分型）。根据国际工作分类，本组Ｔ细胞淋巴瘤所占比例分别是１１％，２７％和１３％。全部病例中ＬＣＡ阳性率９７％。Ｔ细胞抗体ＵＣＨＬ；阳性率（９１％）明显高于ＭＴ１（７３％），且与Ｂ淋巴细胞无交叉反应。４种Ｂ细胞抗体阳性率分别为ＬＮ。（９６％），ＭＢ２（９４％），ＬＮ１（８６％），ＭＢ１（８４％）。ＬＮ２和ＬＮ１对源于滤泡中心细胞淋巴瘤有更强的反应。研究结果表明上述单克隆抗体可有效用于非何杰金氏淋巴瘤石蜡切片的免疫组织化学标记，合理选用单克隆抗体有助于进行免疫学分型。

IMMUNOPHENOTYPIC STUDY OF NON-HODGKIN'S LYMPHOMA IN PARAFFIN SECTIONS

An immunophenotypic study of 65 cases Non-Hodgkin's lymphomas was performed using a panel of monoclonal antibodies (LCA, UCHL1, MT1, MB1, MB2, LN1, LN2) on routinely formalin-fixed, paraffin-embeded tissue sections. There were 50 cases exhibiting Bcell phenotype (77% ) and 11 cases showing T-cell phenotype (17% ). The frequency of Toefl phenotype of the low, intermediate and high grade malignant lymphomas in the international Working Formulation were 11%, and 27% and 13%, respectively. The positive rateof ACL was 97% in this series. The positive rate of T-cell antibody UCHL, (91%) were significantly higher than that of MT, (73%), and the former had no cross-lineage reactivities onB-cell. The positive rate of LN2, MB2, LN1, and MB1 were 96%, 94%, 86% and 84%, successively. LN2 and LN1 reacted more strongly with the follicular center-derived lymphomas.The results suggest that the antibodies described in this study should ho effectively applyed to immunohistochemical staining on the paraffin sections of Non-Hodgkin's lymphomas. Rational selection of monoclonal antibodies is of great help in immunophenotyping.