| 人脐血间充质干细胞分离培养方法的优化 |
| |
| 引用本文： | 张炳强,于丽,石娟,管英俊,马丽,张雪莉.人脐血间充质干细胞分离培养方法的优化[J].中国组织化学与细胞化学杂志,2009,18(2):123-127. |
| |
| 作者姓名： | 张炳强 于丽 石娟 管英俊 马丽 张雪莉 |
| |
| 作者单位： | 1. 潍坊医学院组织学与胚胎学教研室,潍坊,261042
2. 潍坊市妇幼保健院,潍坊,261011 |
| |
| 基金项目： | 山东省教育厅基金，潍坊医学院博士启动基金 |
| |
| 摘 要： | 目的探讨人脐血间充质干细胞（MSCs）体外培养纯化的最佳方法,为脐血MSCs在临床的广泛应用奠定基础。方法无菌条件下采集足月分娩和早产儿脐血,密度梯度离心法分离脐血单个核细胞,比较胎龄、不同培养基、接种密度、首次换液时间对脐血MSCs原代培养过程的影响,通过免疫荧光方法检测表面标记物的表达情况,观察脐血MSCs的生物学特性。结果足月分娩脐血,采用MesencultTM培养基,以5×106/cm2的密度接种,首次换液时间为7d时,脐血MSCs原代培养成功率较高。相同培养条件下,早产儿脐血培养成功率高于足月分娩脐血。人脐血MSCs强表达CD29、CD44和CD90,不表达造血干细胞表面标志CD34。结论优化筛选到一种合适的人脐血MSCs培养纯化条件。
|
| 关 键 词： | 脐血 间充质干细胞 细胞培养 优化 |
OPTIMIZATION OF THE ISOLATION AND CULTIVATION METHOD OF HUMAN UMBILICAL CORD BLOOD MESENCHYMAL STEM CELLS |
| |
| Zhang Bingqiang,Yu Li,Shi Juan,Guan Yingjun,Ma Li,Zhang Xueli.OPTIMIZATION OF THE ISOLATION AND CULTIVATION METHOD OF HUMAN UMBILICAL CORD BLOOD MESENCHYMAL STEM CELLS[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(2):123-127. |
| |
| Authors: | Zhang Bingqiang Yu Li Shi Juan Guan Yingjun Ma Li Zhang Xueli |
| |
| Institution: | 1Department of Histology and Embryology;Weifang Medical College;Weifang;261042;2Weifang Woman and Infant Nursing Center;Weifang 261011;China |
| |
| Abstract: | Objective To explore the optimal condition of isolation and cultivation of mesenchymal stem cells(MSCs) derived from human umbilical cord blood.Methods Umbilical cord blood was collected from full-term deliveries and preterm infants.The umbilical cord blood mononuclear cells were isolated by density gradient centrifugation with a lymphocyte separation medium.The influence of different gestational ages,culture media,planting densities and the time points of the first medium change on the growth of MSCs were ... |
| |
| Keywords: | Umbilical cord blood Mesenchymal stem cells Cell culture Optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
