| 电镜图像定量分析肺毛细血管碱性磷酸酶在高压氧研究中的应用 |
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| 引用本文： | 徐根兴,袁驾南,董文度.电镜图像定量分析肺毛细血管碱性磷酸酶在高压氧研究中的应用[J].中国组织化学与细胞化学杂志,1995(1). |
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| 作者姓名： | 徐根兴 袁驾南 董文度 |
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| 作者单位： | 南京海军神经生物学研究中心细胞生物学研究室 |
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| 基金项目： | 中国人民解放军总后科研基金 |
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| 摘 要： | 采用碱性磷酸酶（ＡＬＰ）的电镜细胞化学方法，观察在高压氧条件下小鼠肺毛细血管ＡＬＰ活性的分布、活性强度与超微结构改变之间的联系，并用计算机图像定量测量方法对酶活性变化进行了２１个参数的定量分析。文中介绍了多参数图像定量分析方法及计算公式。
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| 关 键 词： | 碱性磷酸酶，图像分析，高压氧，肺 |
APPLICATION OF IMAGE ANALYSIS METHOD OF ALKALINE PHOSPHATASE IN CAPILLARIES OF LUNG IN THE STUDY OF HYPERBARIC OXYGEN |
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| Xu Genxing,Yuan Jianan, Dong Weidu.APPLICATION OF IMAGE ANALYSIS METHOD OF ALKALINE PHOSPHATASE IN CAPILLARIES OF LUNG IN THE STUDY OF HYPERBARIC OXYGEN[J].Chinese Journal of Histochemistry and Cytochemistry,1995(1). |
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| Authors: | Xu Genxing Yuan Jianan Dong Weidu |
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| Abstract: | The localization and changes of alkaline phosphatase(ALP) in the capillaries of lung were studied to investigate the effects of hyperbaric oxygen by using image analysis method.The relationship between the changes of ALP and ultrastructure of capillaries of lung were also studied.21 parameters of measured enzymatic activities were analgsed and their calculating formulae by a computer-controlled image analyser were introduced in this paper. |
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| Keywords: | Alkaline phosphatase Image analysis Hyperbaric oxygen Lung |
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