中国荷斯坦奶牛卵巢卵泡细胞的免疫组织化学研究

目的和方法应用七种特异性抗体(sGCα、sGCβ、nNOS、FOXO1、PDE4、PKB/Akt和Inhibinα)对中国荷斯坦奶牛卵巢卵泡细胞进行免疫组织化学定位。结果表明PKB主要存在于原始卵泡、腔前卵泡和有腔卵泡颗粒细胞,黄体细胞中没有检测到;FoxO1主要位于原始卵泡、腔前卵泡和有腔卵泡颗粒细胞;PDE4仅位于部分有腔卵泡的颗粒细胞;抑制素α、nNOS、sGCα和β存在于各级卵泡的颗粒细胞层,其中sGCα和β主要存在于原始卵泡和腔前卵泡的颗粒细胞。结论这七种蛋白的阶段和细胞特异性表达表明它们与中国荷斯坦卵巢卵泡的发育、闭锁和黄体化过程有着密切的关系。

IMMUNOHISTOCHEMICAL STUDIES ON OVARIAN FOLLICULAR CELLS OF CHINESE HOLSTEIN COWS

Objective and Methods To examine the cellular localization of PKB/Akt, FoxO1, PDE4, inhibin α-subunit, nNOS, sGC α and β subunit proteins in the ovarian follicular cells of Chinese Holstein Cows by immunohistochemistry. Results PKB was localized in the granulosa cells of primordial, preantral and antral follicles, but not in corpora lutea. FoxO1 was mainly localized in the granulosa cells of primary, preantral and antral follicles. PDE4 was only localized in the granulosa cells of some antral follicles. Inhibin α-subunit nNOS, sGC α and β subunits were localized in the granulosa cells of follicles at all stages, but not in corpora lutea, and sGC α and β subunit staining were intense in the granulosa cells of primordial and preantral follicles. Conclusion Above stage- and cell-specific expression patterns of PKB/Akt, FoxO1, PDE4, inhibin α-subunit, nNOS, sGC α and β subunits suggest that these proteins might play important roles in follicular development, atresia and luteinization in the Chinese Holstein Cow.